100% satisfaction guarantee Immediately available after payment Both online and in PDF No strings attached
logo-home
SCYM (ASCP) Review - Section 2 - Panel/Experimental Design Questions and Answers 100% Solved 2024/2025 $10.99   Add to cart

Exam (elaborations)

SCYM (ASCP) Review - Section 2 - Panel/Experimental Design Questions and Answers 100% Solved 2024/2025

 11 views  0 purchase
  • Course
  • ASCP CERTIFICATION
  • Institution
  • ASCP CERTIFICATION

SCYM (ASCP) Review - Section 2 - Panel/Experimental Design Questions and Answers 100% Solved 2024/2025

Preview 1 out of 4  pages

  • August 26, 2024
  • 4
  • 2024/2025
  • Exam (elaborations)
  • Questions & answers
  • ascp certification
  • ASCP CERTIFICATION
  • ASCP CERTIFICATION
avatar-seller
Legitexams
SCYM (ASCP) Review - Section 2 -
Panel/Experimental Design

Adherent Samples - ANSCell lines, some tumors

Liquid Samples - ANSBlood, spleen, bone marrow, fresh marine water

Solid Tissue Samples - ANSTumors, organs, plants

Cons of enzymatic disruption methods - ANSCan strip surface epitopes

How to prevent coagulation - ANSACD, EDTA, heparin, or physically disrupt cells using a
method such as vortexing

How long can whole blood remain at room temperature? - ANS24 hours, then refrigerate for up
to 72 hours

Cells that are too large for flow cytometry - ANSCardiomyocytes and Megakaryocytes

Factors to consider when fixing to preserve the epitope - ANSFixative type, fixation time,
temperature, fluorochrome compatibility, surface marker compatibility

Alcohol fixation cannot be used on these fluorochromes - ANSPE and GFP

Aldehydes - ANSformaldehyde, increases background, destroys epitopes, 4% permeabilizes

Alcohol - ANSEthanol and methanol permeabilizes, alters morphology, nuclear, cell cycle

Harsh Detergents - ANSTriton X-100, NP-40

Gentle Detergents - ANSSaponin, Lysolecithin

Centrifugation Gradients - ANSSeperate samples based on density
Percoll
Ficoll

Do voltage plates push or pull droplets? - ANSPush. They are called "deflection" plates.

Probe Types - ANSAntibodies, viability/DNA dyes, physiological, tracking dyes, fluorescent
proteins

The benefits of buying summaries with Stuvia:

Guaranteed quality through customer reviews

Guaranteed quality through customer reviews

Stuvia customers have reviewed more than 700,000 summaries. This how you know that you are buying the best documents.

Quick and easy check-out

Quick and easy check-out

You can quickly pay through credit card or Stuvia-credit for the summaries. There is no membership needed.

Focus on what matters

Focus on what matters

Your fellow students write the study notes themselves, which is why the documents are always reliable and up-to-date. This ensures you quickly get to the core!

Frequently asked questions

What do I get when I buy this document?

You get a PDF, available immediately after your purchase. The purchased document is accessible anytime, anywhere and indefinitely through your profile.

Satisfaction guarantee: how does it work?

Our satisfaction guarantee ensures that you always find a study document that suits you well. You fill out a form, and our customer service team takes care of the rest.

Who am I buying these notes from?

Stuvia is a marketplace, so you are not buying this document from us, but from seller Legitexams. Stuvia facilitates payment to the seller.

Will I be stuck with a subscription?

No, you only buy these notes for $10.99. You're not tied to anything after your purchase.

Can Stuvia be trusted?

4.6 stars on Google & Trustpilot (+1000 reviews)

67096 documents were sold in the last 30 days

Founded in 2010, the go-to place to buy study notes for 14 years now

Start selling
$10.99
  • (0)
  Add to cart