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Exam (elaborations)

ASCP SCYM study Questions and Answers 100% Solved 2024/2025

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ASCP SCYM study Questions and Answers 100% Solved 2024/2025

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  • August 26, 2024
  • 17
  • 2024/2025
  • Exam (elaborations)
  • Questions & answers
  • ascp certification
  • ASCP CERTIFICATION
  • ASCP CERTIFICATION
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ASCP SCYM study

Sample Processing - ANSOptimized antibody concentration
Defined range of cell #
Define sample handling, time and temperature
Define acceptable time of delay for acquisition

CD2 - ANST cells: NK cells

CD3 - ANST cells

CD4 - ANST helper cells, monocytes (dimly expressed)

CD5 - ANST cells; Some B Cells

CD8 - ANST cytotoxic cells, macrophages

CD10 - ANSImmature T, B Cells, PMN's

CD13 - ANSMonocytes, PMN's

CD14 - ANSMonocytes, PMN's (dimly expressed)

CD16 - ANSNK cells, PMN's, monocytes

CD19 - ANSB Cells

CD20 - ANSB Cells

CD25 - ANSActivated T Cells

CD33 - ANSMonocytes

CD34 - ANSStem/Progenitor Cells

CD45 - ANSLeukocytes

CD56 - ANSNK cells, monocytes (dimly expressed)

Anti-Kappa - ANSB Cells

Anti-Lambda - ANSB Cells

,Tdt - ANSImmature Lymphocytes

Monoclonal Antibody Staining Direct Staining - ANSRapid single step staining
Can use multiple antibodies from the same host

Monoclonal Antibody Staining Indirect Staining - ANSSecondary amplifies single
A few labeled secondaries can detect many primaries

Clinical Applications of Flow - ANS-Instrument Performance
-Integrity of the Specimen
-Quality in Processing of Specimens
-Appropriate Reagents and Controls
-Qualified Technical Staff
-Regulatory requirements from CAP, TJC, CLIA, and the FDA exist to ensure the accurate
reporting of flow cytometric results.

Lymphocytes Subset Enumeration (Immunophenotyping) - ANS2 Tubes: 1-
CD3/CD4/CD8/CD45
2- CD3/CD19/CD16&CD56/CD45
-May include analysis of CD4 and CD8 T Cells, B Cells and NK Cells.
-Results reported as proportion (%) of total lymphocyte population.

CD4 Counts in HIV Infection - ANS-Asses degree of immunodeficiency
-Diagnose of AIDS
-Institution of Anti-Retroviral (ARV) therapy and opportunistic infection (OI) prophylaxis
-Monitoring immune reconstitution

CD4 Immunophenotyping Panels - ANS

2 color - ANSIsotype Ctrl
CD45/CD14
CD3/CD4
CD3/CD8
CD3/CD19
CD3/CD16 + 56

3 Color - ANSCD3/CD4/CD45
CD3/CD8/CD45
CD3/CD19/CD16 + 56

4 Color - ANSCD3/CD4/CD8/CD45
CD3/CD16/CD16 + 56/CD45

, Determination of Absolute Counts - ANS-Subset % X Absolute Lymphocyte Count
-Counting Beads- known concentration present in staining tube or added to tube
-Volumetric Cytometers

Leukemia/Lymphoma Phenotyping - ANS-Important in Diagnosis, Classification and Monitoring
of patients with hematologic malignancies.
-Utility based on the fact that hematopoiesis is characterized by the gain and loss of cell surface
and intracellular antigen during the process of maturation.
-Malignant cells may have a block at an immature stage resulting in abnormal numbers of
immune cells. With a mature phenotype may be present in abnormally high numbers. May have
an aberrant phenotype.

Leukemia/Lymphoma Phenotyping FC - ANS-Analysis with panels of monoclonal antibodies
allows for lineage assignment and sub classification that may have further prognostic
significance.
-The identification of a unique phenotype can also facilitate post-therapy monitoring to assess
residual disease.

Relevance for the Diagnosis and Classification of Hematologic Malignancies - ANS-Expression
of aberrant lineage associated antigen
-Absence of lineage associated antigen
-Asynchronous expression of antigens
-Abnormal level of expression of antigen

Detection of Fetal Hemoglobin - ANS-Detection of fetal hemoglobin containing cells in useful for
the diagnosis and management of feto-maternal hemorrhage
-Traditionally assayed with the Kleihauer-Betke method
-FC offers improved sensitivity (<0.02% fetal RBCs) and reproducibility (CV <15%
-Method: Intracellular staining with a fetal hemoglobin specific monoclonal antibody

CD34 Stem Cell Enumeration - ANS-Present on immature hematopoietic cells
-Expression is lost as cells mature
-Used as a marker to identify and enumerate stem cells in bone marrow and peripheral blood
products for transplantation
-Currently the Gold Standard for assessment of HSC products for transplant

CD3 Transplant Monitoring - ANS-Acute graft rejection is an important complication after solid
organ transplantation that may lead to graft loss
-Severe cases of rejection may be treated with antibodies that deplete T cells from the patient
(OKT3, ATG)
-FC is used to assess the effectiveness of T cell depletion after antibody based anti-rejection
therapy

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