PCB 3023 Exam 2: Transcription and Translation Practice Questions and Answers (100% Pass)

PCB 3023 Exam 2: Transcription and Translation Practice Questions and Answers (100% Pass) does the structure of the DNA (nucleosomes) impact the ability of the RNA polymerase to transcribe genes? - Answer️️ -- yes - tightly bound nucleosomes, it's harder to get to the DNA so there is less expression - DNA is more exposed when the histones aren't packed together so tightly dna transcription vs replication - Answer️️ -- replication synthesizes new (daughter) DNA - transcription of DNA forms RNA what direction does transcription proceed? - Answer️️ -RNA is synthesized 5' to 3' what nucleotides are used in RNA synthesis? - Answer️️ -- adenine - guanine - cytosine - uracil ©SOPHIABENNET@2024/2025 Wednesday, August 21, 2024 12:13 PM Molecular Cell Biology PCB 3023 BROWARD COLLEGE/ UNIVERSITY of SOUTH FLORIDA/ UCF COLLEGE Of Sciences 2 what is a promoter in RNA transcription? why is it important? - Answer️️ -- promoter: a sequence in the DNA where the transcription is initiated - importance: control the binding of RNA polymerase to DNA Transcription steps - Answer️️ -1. Initiation 2. Elongation 3. Termination what is an intron? - Answer️️ -- a segment of a DNA or RNA molecule that does not code for proteins and interrupts the sequence of genes. what is an exon? - Answer️️ -- An expressed sequence of DNA; codes for a protein is it true that one gene codes for one protein? - Answer️️ -- no, genes can code for a number of proteins what happens after mRNA as been transcribed in euks? - Answer️️ -- mRNA is exported to the cytosol for processing which includes: capping of the 5' end, splicing, and polyadenylation of the 3' end what is alternative splicing? how does this impact the protein which will be produced by the transcript - Answer️️ -- mRNA processing ©SOPHIABENNET@2024/2025 Wednesday, August 21, 2024 12:13 PM Molecular Cell Biology PCB 3023 BROWARD COLLEGE/ UNIVERSITY of SOUTH FLORIDA/ UCF COLLEGE Of Sciences 3 events that lead to different combinations of exons being spliced together - the protein will be changed binding proteins - Answer️️ -- stabilize separate strands - TFIIH, TFIID - TBP general transcription factors - Answer️️ -- TATA box in the promoter - binding proteins - RNA polymerases mRNA - Answer️️ -- messenger RNA; type of RNA that carries instructions from DNA in the nucleus to the ribosome tRNA - Answer️️ -- transfer RNA; type of RNA that carries amino acids to the ribosome rRNA - Answer️️ -- ribosomal RNA; type of RNA that combines with proteins to form ribosomes snRNA - Answer️️ -small nuclear RNA; Found only in the nucleus of eukaryotes and functions to remove introns from mRNA