Term 1 of 321
What are the steps in DNA replication?
1. Initiate
2. Elongate
3. Terminate
Deletion in region 1, band 4 of the short arm of chr 16
to intiate replication
inherited marker near gene associated with disease
unknown gene and /or mut
Definition 2 of 321
Primary enzyme involved in replication
Exonuclease activity
Type Iii Restriction enzymes
DNA Polymerase III (Prok)
Clopidogrel (plavix)
Huntington disease (hd)
,Term 3 of 321
What is the genetic abnormality of:
46,XY,del(16p14)
Deletion in region 1, band 4 of the short arm of chr 16
Deletion in region 1, band 4 of the short arm of chr 21
Deletion in region 1, band 3 of the short arm of chr 16
Duplication in region 1, band 4 of the long arm of chr 16
Definition 4 of 321
1. RE digest to fragment DNA
2. Run on gel to separate
3. Soak gel in alkali/NaOH to denature dsDNA
4. Transfer ssDNA fragments to positively charged membrane (blot)
5. Fix to filter by heat (80C) or UV crosslink
6. Incubate (hybridize) blot w/ radioactively labeled ssDNA comp probe
7. Autoradiograph
Branched DNA (bDNA)
Organic isolation method
Southern Blot steps
Components of PCR
Term 5 of 321
Methylation of cytosine bases 5' to the gene will increase or decrease expression?
lower
decrease
higher
increase
,Definition 6 of 321
resistance to bacteriocins, toxic proteins manufactured by bacteria.
Chaotropic agents
Terminal transferase
Genome mutations
Colicinogenic factors
Definition 7 of 321
have both nuclease and methylase activity in a single enzyme. Bind to host-specific DNA that
contains methylated adenines
Type I restriction enzymes
Hybridization
Oxidoreductases
Primase activity
Term 8 of 321
What is the genetic abnormality of:
iso(X)(q10)
Isochormosome comprised of the long arms of the X chr
Translocation of the x chromosome's long arm
Deletion of the x chromosome's long arm
Duplication of the x chromosome's short arm
, Term 9 of 321
Calculate the RNA concentration from the following:
260=0.307 (DF 1:100)
2456 ug/ml = 0.614 abs 20 ug/ml 100 Df
1537 ug/ml = 0.384 abs 60 ug/ml 100 Df
614 ug/ml = 0.153 abs 80 ug/ml 100 Df
1228 ug/mL = 0.307 abs 40 ug/mL 100 DF
Term 10 of 321
Quaternary protein structure
arrangement of amino acids in a linear chain
formation of peptide bonds in a protein
3D structure of a multi sub unit protein
interaction of proteins with lipids
Definition 11 of 321
Blue: Health hazard
Red: Fire hazard
Yellow: Reactivity
White: specific hazard
0 no hazard - 4 severe hazard
Whooping cough
Msds
Hazard label
Auxiliary label
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