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MICR 351 TESTED QUESTIONS WITH ALL ANSWERS AND ALL PRINTED PAGES $13.59   Add to cart

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MICR 351 TESTED QUESTIONS WITH ALL ANSWERS AND ALL PRINTED PAGES

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  • MICR 351

MICR 351 TESTED QUESTIONS WITH ALL ANSWERS AND ALL PRINTED PAGES Northern Hybridization? - Answer-mRNA-cDNA hybridization to find gene of interest using DNA probe to identify which bond holds gene of interest Western Hybridization? - Answer-Antibody-protein hybridization, where antibodies bind...

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  • August 29, 2024
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  • 2024/2025
  • Exam (elaborations)
  • Questions & answers
  • MICR 351
  • MICR 351
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MICR 351 TESTED QUESTIONS
WITH ALL ANSWERS AND ALL
PRINTED PAGES

Northern Hybridization? - Answer-mRNA-cDNA hybridization to find gene of interest
using DNA probe to identify which bond holds gene of interest

Western Hybridization? - Answer-Antibody-protein hybridization, where antibodies bind
to a specific protein bond that contains target protein that produces antibody
- used in vaccines and other tests, such as pregnancy tests

Functional genomics - Answer-Microarrays and RNA-sequence that rely on ability to
make short, single stranded DNA

What helps to create short, single stranded DNA? - Answer-Oligonucleotides or primer
synthesis

What is site directed mutagenesis? - Answer-Altering of a specific amino acid by
altering base changes on DNA/gene leading to proteins with altered activities

What technique can be used to amplify multiple copies of genes from few copies using
two primers? - Answer-Polymerase Chain Reaction with 2 oligonucleotides

What are the tree steps per PCR cycle? - Answer-1. Denature at 94ºC
2. Anneal at 50-60ºC
3. Extension at 68-72ºC

What type of PCR is more accurate? Real-time or reverse transcriptase? - Answer-
Real-time PCR

What is the difference between Sangerʻs dideoxy sequencing vs. dyedeoxy
sequencing? - Answer-Sangerʻs Dideoxy:
- one primer that anneals and extends
- build-up of sequence
- dideoxy nucleotide lacks 2ʻ an 3ʻOH, needs a 3ʻ OH to add on next base on

Dyedeoxy:
- includes color dye to dye each base a specific color
- reads 100 bases only
- takes a long time

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