ACS Biochemistry Final Exam Study Questions and Answers UPDATED 2024
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Course
ACS Biochemistry
Institution
ACS Biochemistry
pKa (chapter 3) - measure of the tendency of a group to give up a proton (acidity); tendency decreases tenfold as pKa increases by one unit
thioester bond (1) - compounds with functional group C-S-CoA-C (eg, in acetyl-CoA)
zwitterion (3) - dipolar ion with both positive and negative groups bu...
ACS Biochemistry Final Exam Study
Questions and Answers UPDATED 2024
BESTZONE
ACS BIOCHEM
8/29/24
, ACS Biochemistry Final Exam
pKa (chapter 3) - measure of the tendency of a group to give up a proton (acidity);
tendency decreases tenfold as pKa increases by one unit
thioester bond (1) - compounds with functional group C-S-CoA-C (eg, in acetyl-CoA)
zwitterion (3) - dipolar ion with both positive and negative groups but overall neutral
charge; can act as either an acid or base
Isoelectric point (pI) (chapter 3) - the characteristic pH at which the net electric charge is
zero
SDS - Sodium Dodecyl Sulfate (chap 3) - detergent used to unfold proteins and give
them uniform negative charge
SDS Page (3) - chromatography used to separate proteins based on mass. light
proteins travel fast than heavier ones
PCR - Polymerase chain reaction (3) - copies DNA multiple times to increase sample
size
Isoelectric focusing (chapt 3) - procedure used to determine the isoelectric pt (pI) of a
protein. Protein migrates through gel until pH = pI (net charge = 0)
Two-Dimensional Electrophoresis (3) - combines isoelectric focusing and SDS
electrophoresis; separates proteins by both molecular weight and pI
specific activity (3) - number of enzyme units per mg of total protein (a measure of
enzyme purity)
activity (3) - total units of a certain enzyme in a solution
ACS Biochemistry Final Exam
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