MICR 2123 Exam 1 Questions With Correct Answers
2 techniques to obtain a pure culture - Answer-1) Streak Plate
2) Spread Plate
/.4 Growth Stages - Answer-1) Lag: getting used to the environment and preparing to grow.
2) Log: exponential growth.
3) Stationary: Can't grow any more.
4) Dea...
2 techniques to obtain a pure culture - Answer-1) Streak Plate
2) Spread Plate
/.4 Growth Stages - Answer-1) Lag: getting used to the environment and preparing to
grow.
2) Log: exponential growth.
3) Stationary: Can't grow any more.
4) Death: Nutrients run out and microbes start to die.
/.Acidophiles - Answer-pH (0-5)
/.Active ABC Transporters - Answer-Concentrated in cell. Energy comes from ATP
hydrolysis. ATP- Binding Cassette.
Example: Uptake, Efflux
/.Alkaliphiles - Answer-pH (9+)
/.Antiport - Answer-Coupled active transport where the two molecules move in different
directions.
/.Antisepsis - Answer-NOT sterilization; killing or removing pathogens from living
tissues.
/.Antiseptics - Answer-Chemical agent that kills microbes on living tissues.
/.Barophiles - Answer-High pressure (1000 atm)
/.Biofilms - Answer-Adhere to a surface. Count as microbes. Antibiotic resistant because
it can build thickness. In infections.
/.Biogenesis - Answer-Living organisms come from pre-existing living organisms.
/.Both ABC transporters and group translocation transporters require energy
expenditure. This is manifested in the form of ATP hydrolysis for both kinds of
transporters. (T/F) - Answer-False
, /.Cell Membrane - Answer-Made of proteins (transport) and a phospholipid bilayer
(Semi-permeable to small, uncharged molecules).
/.Cell Wall - Answer-Shape cell and helps resist turgor pressure. Made of peptidoglycan
(alternating NAM and NAG) and the peptide cross-links (amino acids.)
/.Chemical Agents - Answer-Kill microbes, especially when physical agents can not.
Includes disinfectants and antiseptics
Need contact time to work!
Examples: alcohol, chlorine, iodine
/.Complex media - Answer-Nutrient rich, but poorly defined.
Example: Yeast extract
/.Coupled Active Transport - Answer-Move two molecules. Go to driving ion.
Example: Symport, Antiport
/.Cytoplasm - Answer-Gel-like dense filler that is 70% water. Contains the nucleoid.
/.Dark-Field (Object, Field, Light) - Answer-Object: Halos of Light
Field: Dark
Light: Scattered
/.Defined media - Answer-Minimal exact amounts, synthetic.
All the nutrients and parts of it are defined.
/.Detection - Answer-To be certain and aware that something is there.
/.Differential media - Answer-Two species grow equally well, but display different
phenotypes.
/.Differential Stains - Answer-A type of stain that lets you tell one type of cell from the
other. Can stain one cell and not others and can stain different cells with different
colors. Examples: Gram, acid-fast, spore, and negative stain.
/.Disinfectants - Answer-Chemical agent that kills microbes on inanimate objects.
/.Disinfection - Answer-NOT sterilization; killing or removing pathogens from inanimate
objects.
/.Electron microscopy - Answer-Uses beams of electrons, >5 nm (extremely small)
Examples: SEM, TEM
/.Endospores - Answer-Contain DNA and go dormant (starvation). Highly resistant to
heat. When conditions become favorable again, they can germinate vegetative
(sporulation) cells as a last effort.
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