BCHM 270 MIDTERM TEST QUESTIONS WITH CORRECT ANSWERS
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Course
BCHM 270
Institution
BCHM 270
BCHM 270 MIDTERM TEST QUESTIONS WITH CORRECT ANSWERS
Intramolecular disulfide bond - Answer-2 C
(1 C can form disulfide bonds with other C groups)
Phosphorylated - Answer-S, T, Y
Alpha helix breakers - Answer-P
Bulky clusters: Y, W, L, V, F
Charged clusters: K, R, H, D, E
Ionizable si...
BCHM 270 MIDTERM TEST QUESTIONS
WITH CORRECT ANSWERS
Intramolecular disulfide bond - Answer-2 C
(1 C can form disulfide bonds with other C groups)
Phosphorylated - Answer-S, T, Y
Alpha helix breakers - Answer-P
Bulky clusters: Y, W, L, V, F
Charged clusters: K, R, H, D, E
Ionizable side chains - Answer-R, K, Y, C, H, E, D
Scurvy - Answer-Caused by impaired hydroxylation of lysine and proline causing
unstable triple helices due to vitamin C deficiency.
Prion disease - Answer-Proteins are refolded from α-helix into β-sheet form, producing
long insoluble fibrils and a loss of function.
Alzheimer's disease - Answer-Abnormal cleavage of the hydrophobic section of a
protein, which causes aggregation into long, insoluble neurotoxic fibrils.
Ostegenesis imperfecta - Answer-Mutation of Gly-X-Y repeating sequences into bulky
amino acids, preventing assembly of helices into a mature collagen fiber (brittle bone
disease).
Respiratory acidosis - Answer-Caused by a sharp increase of carbon dioxide in the
blood.
Sickle cell anemia - Answer-The aggregation of a mutated hemoglobin protein caused
by the substitution of glutamic acid to valine.
Ehlers-Danlos syndrome - Answer-Proteins are refolded from α-helix into β-sheet form,
producing long insoluble fibrils and a loss of function.
Oxidoreductase - Answer-Transfer electrons between molecules; catalyze oxidation-
reduction reactions.
Transferase - Answer-Transfer functional groups between molecules.
Hydrolyase - Answer-Cleaves molecules by the addition of water.
, Lyase - Answer-Adds atoms or functional groups to a double bond or removes them to
form double bonds.
Isomerases - Answer-Move functional groups within a molecule.
Ligases - Answer-Join two molecules in a reaction powered by ATP hydrolysis.
Spontaneous - Answer-Negative ΔG
Requires additional energy - Answer-Positive ΔG
Equilibrium - Answer-ΔG = 0
Exergonic reaction - Answer-Releases energy
Endergonic reaction - Answer-Absorbs energy
Competitive inhibitor - Answer-Binds to substrate-free active site
Vmax doesn't change
Km increases
Uncompetitive inhibitor - Answer-Binds to substrate bound enzyme
Vmax decreases
Km decreases
Noncompetitive inhibitor - Answer-Equal affinity to bind to both substrate bound and free
enzyme
Vmax decreases
Km doesn't change
Mixed inhibitor - Answer-Binds to both substrate bound and free enzyme, but favours
one over other
Vmax decreases
Km increases or decreases
Protein synthesis:
a) Is the transcription of DNA to protein
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