FRNSC421W - QUIZ 4 WITH 100%
CORRECT ANSWERS
STR fluorescence incorporation - Answer️️ -incorporates fluorescent dyes on
PCR primers as labels for detection of target alleles
- one of two primers at each locus is labeled
- only one ssDNA fragment will be detected for each allele
- allows ...
- each DNA fragment sized by an ILS, all run in the same capillary with different
dyes
- migration anomalies often seen on edge of gel; move faster because of increased
heat, i.e. 'curling bases'
- detection window excites dyes, CCD camera captures emission as a picture
- creates 'standard' for fragments to be sized by scan # (x-axis) vs size in bps (y-
axis), where larger DNA fragment has larger scan # (more pictures taken)
local southern method - Answer✔️✔️-uses a virtual standard curve using three data
points
1) generate virtual standard curve using 2 ILS fragments above and 1 ILS fragment
below where unknown DNA falls on curve, extrapolate bp value
2) generate virtual standard curve using 1 ILS fragment above and 2 ILS fragments
below where unknown DNA falls on curve; extrapolate bp value
3) average two values
multi-color fluorescence genotyping - Answer✔️✔️-the ILS run with the Fusion
allelic ladder allows for ladder fragments to be sized and placed into sizing/allele
bins
- unknown DNA fragments sized with their own ILS can be placed into bins and
designated an allele number
- unshaded bins are partial repeats
- fragments of DNA with all six dyes are run through the same capillary; software
separates fragments by dye color
allele designations - Answer✔️✔️-based on ISFG rules
2
The benefits of buying summaries with Stuvia:
Guaranteed quality through customer reviews
Stuvia customers have reviewed more than 700,000 summaries. This how you know that you are buying the best documents.
Quick and easy check-out
You can quickly pay through credit card or Stuvia-credit for the summaries. There is no membership needed.
Focus on what matters
Your fellow students write the study notes themselves, which is why the documents are always reliable and up-to-date. This ensures you quickly get to the core!
Frequently asked questions
What do I get when I buy this document?
You get a PDF, available immediately after your purchase. The purchased document is accessible anytime, anywhere and indefinitely through your profile.
Satisfaction guarantee: how does it work?
Our satisfaction guarantee ensures that you always find a study document that suits you well. You fill out a form, and our customer service team takes care of the rest.
Who am I buying these notes from?
Stuvia is a marketplace, so you are not buying this document from us, but from seller OliviaWest. Stuvia facilitates payment to the seller.
Will I be stuck with a subscription?
No, you only buy these notes for $9.99. You're not tied to anything after your purchase.
