Class notes
Class Notes: Lecture 3 of M118L Course at UCI
- Institution
- University Of California - Irvine
Notes cover lecture 3A, 3B and 3C videos of M118L course at University of California, Irvine. Includes lecture 3's practice problems.
[Show more]
Seller
Follow
Content preview
Lecture 3 (covers videos A, B, & C) Abbreviations:Ex. = for example,
Tuesday, January 19, 2021 6:23 PM b/w = between
b/c = because
w/ = with
v. = versus
A. Lab Problem: future direction (follow-up expt) for the biofuels paper? INC = increase
○ Must be related to the results of the paper and be specific(research questions,
steps). C. Analyzing the Biofuel Paper
○ Do NOT do the same procedure w/ another organism! • Main question: is it possible to generate yeast that show INCed tolerance to glucose and
• Antibiotics: ethanol levels?
○ Broad v. narrow(works against certain bacteria) spectrum • Chose to modify TFs (transcription factors) b/c the alterations would affect many genes. ->
○ Static(prevent bacterial growth) v. cidal(kill the bacteria) Better chance at producing an ethanol- and glucose- tolerant mutant.
○ Antibiotics shut down specific pathways in the bacterial target cell: cell wall, protein • Used PCR mutagenesis to modify TFs, and added mutants to WT yeast. Evaluated who
synthesis, nucleic acid, metabolic pathway inhibitors. would survive in an ethanol-glucose rich dish.
○ Fungal genes are much more similar to ours than bacteria. -> Much harder to • Fig. 1B: evaluates survival of mutated TFs in high glucose environment.
target fungal genes specifically/much easier to get cross-reactivity with antifungal • OD: Optical Density. Measures of bacterial/yeast growth.
medicine. • Fold Improvement: how much better is the experimental group growth compared to the
○ Significant INC in the use of antibiotics since 90s -> INC in antibiotic resistance. control group(i.e. WT)?
• Bacteria become resistant through: ○ Measures the OD of SPT15-300 over the WT and the OD of TAF25 over the WT.
○ Spontaneous Mutation: promoter mutations(i.e. overexpression of ribosomal • At 20g/L glucose, the spt15-300 mutant is at 7.8 fold improvement. -> SPT15-300 OD /
proteins), ORF mutation(Open Reading Frame, i.e. structure of ribosomal protein is WT OD = 7.8regular
altered). MODIFYING EXISTING GENOME! • Hypothesis: TPS1 promoter will have an overall higher ethanol production than the SPT15
○ Resistance plasmids that allow acquisition of new genes. Proteins that and PDC mutant strain. PDC promoter will increase in ethanol production as glucose
breakdown/modify antibiotics, drug pumps, new versions of target protein w/ levels increase.
different structure. ADDING SOMETHING NEW!
LECTURE PRACTICE PROBLEMS
The benefits of buying summaries with Stuvia:
Guaranteed quality through customer reviews
Stuvia customers have reviewed more than 700,000 summaries. This how you know that you are buying the best documents.
Quick and easy check-out
You can quickly pay through credit card or Stuvia-credit for the summaries. There is no membership needed.
Focus on what matters
Your fellow students write the study notes themselves, which is why the documents are always reliable and up-to-date. This ensures you quickly get to the core!
Frequently asked questions
What do I get when I buy this document?
You get a PDF, available immediately after your purchase. The purchased document is accessible anytime, anywhere and indefinitely through your profile.
Satisfaction guarantee: how does it work?
Our satisfaction guarantee ensures that you always find a study document that suits you well. You fill out a form, and our customer service team takes care of the rest.
Who am I buying these notes from?
Stuvia is a marketplace, so you are not buying this document from us, but from seller PrinceAlixD. Stuvia facilitates payment to the seller.
Will I be stuck with a subscription?
No, you only buy these notes for $2.99. You're not tied to anything after your purchase.
Can Stuvia be trusted?
4.6 stars on Google & Trustpilot (+1000 reviews)
62890 documents were sold in the last 30 days
Founded in 2010, the go-to place to buy study notes for 14 years now