100% satisfaction guarantee Immediately available after payment Both online and in PDF No strings attached
logo-home
Previously searched by you
Previously searched by you
logo
Class Notes: Lecture 7 of M118L Course at UCI $2.99   Add to cart
Quickly navigate to
Preview
  2.  
  3. University Of California - Irvine
  4.  
  5. Experimental Microbiology Lab (M118L)

Class notes

Class Notes: Lecture 7 of M118L Course at UCI
 6 views  0 purchase

Notes cover lecture 7A video of M118L course at University of California, Irvine. Covers concepts like comparing aged to un-aged cells in microbiology lab experiments.

Preview 1 out of 3  pages

Document information

  • September 1, 2024
  • 3
  • 2020/2021
  • Class notes
  • Brian sato
  • All classes

Subjects

Written for

All documents for this subject (8)

Seller

avatar-seller
PrinceAlixD

Content preview

Lecture 7 (covers video A)
Tuesday, February 16, 2021 11:03 PM




• Aged cells are compared to unaged--same strain, only grown for 1-2d, fresh and metabolically-active-- cells.


Once aged cells show growth advantage, they exhibit the GASP phenotype.


• Hypothesis: is the GASP phenotype present? Does it differ in different media?


• Pipetting expt: determining how many colony-forming units (CFUs) will come up if a mL of culture is diluted from


0,3,6,9 days? CFU is a measurement of concentration.


○ Each spot represents a 10-fold dilution.


○ In earlier spots, there's so much growth cells are merging onto one another-- must look at spot 5+.


○ Aged cells are grown in their LTSP-- 90% of the cells in the growth culture are dead. Shining light onto a


culture full of dead cells and measuring the OD will not determine how many aged cells are still alive/can


grow.


○ Spot 1 is 10ul of the diluted culture (10ul culture + 90ul LB). This means it contains 1ul of the original


culture. ->


○ Spot 1: CFU/10^-3ml = CFU * 10^3/ml

The benefits of buying summaries with Stuvia:

Guaranteed quality through customer reviews

Guaranteed quality through customer reviews

Stuvia customers have reviewed more than 700,000 summaries. This how you know that you are buying the best documents.

Quick and easy check-out

Quick and easy check-out

You can quickly pay through credit card or Stuvia-credit for the summaries. There is no membership needed.

Focus on what matters

Focus on what matters

Your fellow students write the study notes themselves, which is why the documents are always reliable and up-to-date. This ensures you quickly get to the core!

Frequently asked questions

What do I get when I buy this document?

You get a PDF, available immediately after your purchase. The purchased document is accessible anytime, anywhere and indefinitely through your profile.

Satisfaction guarantee: how does it work?

Our satisfaction guarantee ensures that you always find a study document that suits you well. You fill out a form, and our customer service team takes care of the rest.

Who am I buying these notes from?

Stuvia is a marketplace, so you are not buying this document from us, but from seller PrinceAlixD. Stuvia facilitates payment to the seller.

Will I be stuck with a subscription?

No, you only buy these notes for $2.99. You're not tied to anything after your purchase.

Can Stuvia be trusted?

4.6 stars on Google & Trustpilot (+1000 reviews)

67096 documents were sold in the last 30 days

Founded in 2010, the go-to place to buy study notes for 14 years now

Start selling
$2.99
  • (0)
  Add to cart