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BIO 171 M3 Module 3 Review Essential Microbiology with Lab Dalbey Portage Learning
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BIO 171 M3 Module 3 Review Essential Microbiology with Lab Dalbey Portage Learning
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A nanometer is defined as: - 10 -9 or one-billionth of a meter
True or False: A nanometer is longer than a micrometer - False
A nanometer is 1000x smaller than a micrometer
Resolution and contrast are two critical factors that influence your ability to see an object.
Explain each. - Resolution refers to the distance between two objects at which the
objects can still be seen as separate. Poor or low resolution means two or more objects
may appear as one.
Contrast on the other hand is the difference in light absorbance between two objects.
Poor contrast gives a high background and makes the visualization of multiple objects
difficult. For instance, trying to identify 2 dark colored objects at
night (low light = low contrast) versus the same 2 objects in the middle of
a sunny afternoon (bright light against 2 dark objects = high contrast).
Assuming a fixed ocular, identify the part of the microscope you would adjust to enhance
the magnification of a sample. - Objective
Only the oculars (eyepiece) and the objectives contribute to the magnification of the
sample. Since the eyepiece
is fixed, only the objectives could be altered.
Assuming a constant (non-adjustable) light source power, identify the part of the
microscope you would adjust to limit the amount of light entering the microscope. - Iris
diaphragm
What is the total magnification on (relative to your eye) of a sample imaged with a 60x
objective and a 10x eyepiece? Show your math. - 60x objective x 10x ocular = 600x
magnification
True or False: Staining is often required to image a cell that is adherent and flat (thin). -
True
Adherent, flat and unstained cells are almost invisible due to the limits on both
resolution and contrast. Therefore, cell staining is often required to adequately image
the sample.
True or False: A cell that is adherent, flat (thin), and unstained is easily identified using
bright field microscopy - False: Adherent, flat cells are almost invisible due to the limits
on both resolution and contrast
Which of the following could be seen clearly by the unaided eye? Select all that apply.
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