BCH5413 Test Questions Solved 100% Correct | Latest Update
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Course
BCH5413
Institution
BCH5413
In the development of knockout mice, it is critical to genotype the mice to
determine if the gene of interest is actually knocked out. Below is a figure for the
primer locations for triplex PCR. Which of the following PCR results would
represent true knockout mice? - Answer One band of relativel...
BCH5413 Test Questions Solved 100% Correct |
Latest Update
In the development of knockout mice, it is critical to genotype the mice to
determine if the gene of interest is actually knocked out. Below is a figure for the
primer locations for triplex PCR. Which of the following PCR results would
represent true knockout mice? - Answer One band of relatively lower molecular
weight
* The primer pair for the knockout gene produces a smaller fragment than the
endogenous gene. If both genes are replaced with PGK-NEO, PCR will produce one
band of a relatively smaller (lower molecular weight) size.
In the mammalian shuttle vector pcDNA3.1, the purpose of the neomycin
resistant gene is to... - Answer To select for mammalian cells that were
successfully transfected.
polylinker - Answer to match vector and insert restriction sites
antibiotic resistance gene - Answer to select against bacteria that did not take up
the plasmid
origin of replication - Answer to make copies of the plasmid once in cells
LacI gene - Answer to regulate expression of your gene of interest
LacZ gene - Answer to screen for plasmids that contain the gene of interest
, Type of information found in a cDNA library - Answer The genes that are
expressed in a particular tissue or cell type. cDNA is reverse transcribed from the
RNA that was expressed in a tissue or cell type.
the purpose of electromobility shift assay... - Answer determine if a specific
protein binds to a specific DNA in vitro
If a protein of interest binds to a labeled oligonucleotide, it will increase the
molecular weight of the fragment and shift it to a higher position in an acrylamide
gel.
the goal of ChIP-Seq is to identify... - Answer All DNA sequences bound to a
protein of interest
in nonsense mutation - Answer a single base change creates a stop codon instead
of an aa
the advantage of Cassette Mutagenesis over QuikChange... - Answer in Cassette
Mutagenesis no PCR is necessary, so there is no possibility of replication errors.
in the example of stable transfection of sFRP1 in human kidney cancer cells,
match each technique to the rationale for including the technique to the study:
microarray, cloning into a shuttle vector, stable transfection, western blot,
measure tumor volume in mouse model - Answer -microarray: identify the genes
that are overexpressed in kidney cancer cells. sFRP1 was one of those genes
-cloning into a shuttle vector: create a sFRP1 construct that can be maintained in
E.coli and mammalian cells
-stable transfection: create a cell line that permanently express sFRP1
-western blot: determine that the sFRP1 protein is overexpressed from a plasmid
construct on a stable cell line
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