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ACS Biochem Final Test Questions with correct Answers
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ACS Biochem Final Test
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ACS Biochem Final Testnet reaction for glycolysis - answer2x Pyruvate + 2x ATP + 2x NADH per glucose
molecule
what step produces 2x NADH in glycolysis? - answerG3P -> 1,3 BPG
What enzyme/pathway does ATP inhibit? – answer A high level of ATP inhibits the
enzyme PFK-1 by decreasing its affinity for fructose 6-phosphate. AMP diminishes and
citrate enhances the inhibitory effect of ATP.
The NADPH produced by HMP is used for - answer reductive biosynthesis (anabolic)
how do competitive inhibitors affect Km and Vmax? - answer increases Km, doesn't
change Vmax
how do noncompetitive inhibitors affect Km and Vmax? – answer Km doesn't change,
Vmax lowers
SDS-PAGE - answer Type of chromatography used to separate proteins based on
mass. SDS binds to the proteins, giving them a negative charge cancelling the effect of
charges from the individual amino acids. Lighter proteins travel faster than heavier
ones.
PCR - answerpolymerase chain reaction- DNA is copied multiple times to produce many
copies of the original molecules helpful when there's only a small DNA sample
Arginine (pka) - answerPositively charged R groups (pKa=12.5)
Aspartic Acid (pka) - answerNegatively charged R groups (pKa=3.9)
Cysteine (pka) - answerPolar, uncharged R groups (pKa=8.3)
Glutamic Acid (pka) - answerNegatively charged R groups (pKa=4.3)
Histidine (pka) - answerPositively charged R groups (pKa=6.0)
Lysine (pka) - answerPositively charged R groups (pKa=10.5)
Tyrosine (pka) - answerPositively charged R groups (pKa=10.1)
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