Explain the Beckman-Coulter and Sysmex Hematology analyzers in terms of principle [electrical impedance]
Beckmann-Coulter:
- STKS
> counts >6000 cells/s, 5 part diff, 22 parameters
- GENS
> all features of STKS in addition to CD4/CD8 counts and slidemaker
- MAXM
> 5 part diff...
, > Uses cyanide-free sodium lauryl sulfate (SLS)
> Reagent lyses RBCs and WBCs in patient sample
> This oxidizes the heme group allowing for binding of the SLS and forming a
colored compound where absorbance can be measured and is proportional to the
hemoglobin in sample
- Cellavision DM1200
> displays image of cells on blood smear
> performs differential count of. WBCs
> characterizes RBC morphology and platelet estimation
Distinguish among measured and calculated parameters of Beckman-Coulter
Hematology analyzers - ANSWER- Measured:
WBC count and differential
RBC count
PLT count
Hemoglobin
MCV
Calculated:
MCH
MCHC
RDW
PDW
Hematocrit
Calculate the MCV, MCH, MCHC given pertinent data - ANSWER- MCV= HCT% x
10/ RBC count
MCH= Hgb x 10/ RBC count
MCHC= Hgb x 100/ Hct %
Compare and contrast a 3-part versus a 5-part WBC differential - ANSWER- 3 part
diff:
- granulocytes
- monocytes
- lymphocytes
5 part diff:
- segmented neutrophils
- eosinophils
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