CMB2001 L01 Exam Questions With
Verified Answers.
What is the span of the core/basal region of the eukaryotic promoter? - answer-30 to +30nt
What elements are in the core region of the eukaryotic promoter? - answerTATA box
Inr (initiator)
DPE (downstream promoter element)
BRE (TFIIB recogniti...
What is the span of the core/basal region of the eukaryotic promoter? - answer✔✔-30 to +30nt
What elements are in the core region of the eukaryotic promoter? - answer✔✔TATA box
Inr (initiator)
DPE (downstream promoter element)
BRE (TFIIB recognition element)
Describe the TATA box consensus sequence - answer✔✔-31 to -26nt, TATAWAWR
Describe the Inr sequence - answer✔✔-2 to +4nt, YYANWYY with an A at +1
What is observed in core promoter CpG islands without obvious TATA and Inr sequences? -
answer✔✔Lower transcription rate, initiation at multiple start sites
What is the difference between a distal and proximal regulatory promoter element? -
answer✔✔Proximal = within 1kb of the start site
Distal = 1kb or more away from the start site
How do you identify promoter elements via sequence comparison? - answer✔✔Analyse base
frequency within many genes to compare and identify a consensus sequence
How do you identify promoter elements via reporter analysis? - answer✔✔Insert a reporter gene
downstream of a regulatory sequence within a plasmid and measure expression of the reporter
protein.
The impact of mutations and control, induction, timing and location of transcription can be
measured by this.
Describe RNA polymerase I - answer✔✔Found in the nucleolus, it transcribes 28S, 18S and
5.8S rRNA
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