OSMT- Laboratory safety Questions And Answers
acute radiation - Answer-large doses over a short period causes
-skin disorders
-bone marrow
-digestive system
-neuromuscular system
/.administrative controls - Answer--rules and processes, safe work procedures and training
ex:
-haz...
acute radiation - Answer-large doses over a short period causes
-skin disorders
-bone marrow
-digestive system
-neuromuscular system
/.administrative controls - Answer--rules and processes, safe work procedures and
training
ex:
-hazardous assessments
- procedures designed to reduce risk
-signage with hazard identifiers, symbols or pictograms
-implement routine practices and precautions
-workers training
-enforcement of safety rules
-hygiene and housekeeping
-post exposure protocols to minimize harm
/.are droplets considered less of a respiratory hazard and what PPE should workers
wear - Answer-they are less of a hazard because they settle out to the atmosphere very
quickly due to large size
workers should wear surgical masks to protect them from one meter or more to the
source
/.are hazards the same as risks? - Answer-no
/.are masks considered respirators? - Answer-no they are not - they only protect against
direct contact
/.Autoclaves function effectively through proper control of (5) - Answer-1) pressure
2) temp
3) moisture content
4) time
5) contact
/.biological spills on a person - Answer--if there are aerosols, remove person to a
different part of the lab
,-remove contaminated clothing
-flush skin with shower with disinfectant soap and water
/.broken tubes in centrifuge - Answer--turn off
-don ppe
-inform others and let centrifuge sit for 30 min
-remove lid and broken tubes with forceps
-place unbroken capped specimen in disenfectant
-wipe the bowl with disinfectant and water twice and dry
-remove sealed bucket in BSC
-if any tubes are broken, leave in bucket and replace lid loosly and autoclave the entire
contents or place in disinfectant
/.BSC - look at manual - Answer-pg 51 of safety manual
/.BSC must comply with which organization and be certified by which organization -
Answer--Canadian Biosafety Standard
-Certified by The National Sanitation Foundation International Standard/American
National Standard 49 Biosafety Cabinetry
/.chemical spill - only preformed by qualified personal - Answer--select PPE
-refer to SDS sheet for information
-determine method of clean up
-when applying absorbents or neutralizers circular motion outwards going in
-wash area
-package all contaminated materials into appropriate containers
/.chemical spills- leak of volatile toxic , corrosive or flammable chemicals - Answer--turn
off hot plates or other ignition sources if safe
-get an keep everyone out of lab
-establish barriers and post warning signs to prevent re entry into spill area
-make sure fume hoods or other local exhausts are working
-advise the ventilation department to take steps to eliminate possibility of air re-
circulation
/.definition of containment levels - Answer--the minimum physical features and
operational practices needed for the safe handling and storage of biohazards within an
identified area such as the lab
, /.does the size and shape of the hepa filters depend on what kind of BSC it is? -
Answer-yes it does,
/.duties of employer - Answer-educating and training workers for safe use
ensuring products are properly labeled and provide current SDS sheets for workers
/.duties of the supplier - Answer-determines which products are hazardous , provide
appropriate labeling and SDS sheets
/.duties of worker - Answer-attend programs and training
using control measures
/.electrical precautions - Answer--know where the circuit breakers and fuse box are
-do not use extension chords
-use circuits with ground faults to eliminate human risk
-use licensed eletricians
-never remove ground pin
-use only electrical equipt with CSA labels
-use only class C extinguishers on electrical fires
/.engineer controls for biological hazards - Answer--eliminate or substitute exposure to
risk
-immunization programs
-improve design of lab
-heating, ventilation and AC systems
-autoclaves and incinerators- decontaminate
-BSC
-self-sheathing needles
-sharp disposal containers
-engineered transport containers
-barrier pipette tips
/.equipt shelves should have what to prevent chemicals from falling - Answer-edge
guards
/.general precautions when handling radioactive materials - Answer--know the types of
materials
-determine shielding requirements
-use PPE
-use shielded storage
-if you use the wrong shielding it can lead to effects that are harmful
-use Plexiglas for shielding not lead
/.handling of crygenics fluids - Answer--do not mix materials
-control ignition sources
-assure proper training
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