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ACS Biochemistry Review Exam 2024 Questions and Verified Answers, 100% Guarantee Pass
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ACS Biochemistry Review Exam 2024 Questions and Verified Answers, 100% Guarantee Pass
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Biochem ACS Review.pdf file:///C:/Users/HP/Desktop/New%20folder/Biochem%20ACS%20RBIOCHEM ACS Review
Questions & Answers
1. What amino acids have nonpolar, aliphatic R groups?
Answer Glycine, Alanine, Proline, Valine, Leucine, Isoleucine, Methionine
2. What amino acids have polar, uncharged R groups?
Answer Serine, Threonine, Cys- teine, Asparagine, and Glutamine
3. What amino acids have aromatic R groups?
Answer Phenylalanine, Tyrosine, and Tryptophan
4. What amino acids have negatively charged R groups?
Answer Aspartate and Gluta- mate
5. What amino acids have positively charged R groups?
Answer Lysine, Arginine, and Histidine
6. What is isoelectric focusing?
Answer Proteins are electrophoresed in a pH gradient gel. Each protein will move in the gel
as long as the protein contains a charge
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,Biochem ACS Review.pdf file:///C:/Users/HP/Desktop/New%20folder/Biochem%20ACS%20R
7. What does SDS do?
Answer It binds to proteins and denatures it. All proteins have same mass/ charge ratio
8. How do you determine the Amino Terminus?
Answer 1. Make a derivative of the N-terminus with a marker molecule
2. Hydrolyze the peptide
3. N-terminal AA is identified by chromatography- modified amino acid will elute differently
than unmodified AA
9. What molecule does Edman Degradation use?
Answer Phenyl Isothiocyanate (PTH)
10. What does Edman Degradation do?
Answer It removes one amino acid at a time.The limit is 50 amino acids. After 50 amino acids,
the polypeptide must be hydrolyzed into smaller fractions
11. Where does Cyanogen Bromide cleave?
Answer Cleaves only on the caryboxyl side of Methionine residues
12. Where does Trypsin cleave?
Answer Trypsin cleaves on the carboxyl side of positive residues such as Arginine and Lysine
13. What happens in Disulfide Position?
Answer It is a diagonal electrophoresis.
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