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Base Excision Repair questions and answers.
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Base Excision Repair questions and answers.
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Base Excision Repair questionsand answers
What is the first step in Base Excision Repair - answer The excision
of a single base
A thymine (T) base is taken off the strand while the guanine (G)
base is still left on. What enzyme takes off the thymine (T) base? -
answer DNA Glycosylase
Why does DNA glycosylase cleave off the thymine (T) base only and
not the guanine (G) base? - answer Becasue this mismatch was
generated by a C to T translation
+ Cells will automatically recognize the thymine residue as the
mutated on, not the guanine
What specifically does the DNA glycosylase enzyme do to the
thymine (T) base? - answer Cleaves the N-glycosidic bond aka the
bond that links the sugar to the base
Another enzyme is recruited that cuts the phosphodiester linkage,
resulting in an exposed 3' hydroxyl group (OH-) and a 5' phosphate
group (PO^4-) is known as...? - answer AP endonuclease I (APEI)
After AP endonuclease, there is another enzyme that is recruited.
What is this enzyme known as? - answer AP lyase (aka Apurinic
lyase)
+ Apurininc meaning "without purine/pyrimidine"
What does AP lyase do in this process? - answer AP lyase works with
the associated DNA polymerase I to remover the 5' phosphate group
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