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BACE - Version A - Technical Skills & Applications questions and answers.
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BACE - Version A - Technical Skills & Applications questions and answers.
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BACE - Version A - TechnicalSkills & Applications questions
and answers
Why must technicians practice aseptic technique when culturing
cells? - answer To ensure they are growing pure cultures of their
desired cells.
Which of the following techniques destroys all microorganisms? -
answer Sterilization
When using a microscope on the highest power objective, which
focus knob should you use? - answer Fine
A solution with a pH of 3.0 is how many times more acidic than a
solution with a pH of 6.0? - answer 1,000
Salt (such as sodium chloride or sodium acetate) is commonly used
in procedures to extract plasmid DNA. Which of the following best
describes the role of the salt? - answer The positively charged
sodium ions neutralize the negative charge on the DNA.
Why is plasmid DNA is purified using anion exchange column
chromatography? - answer DNA is negatively charged, binds to the
positively charged resin, and is eluted with increasing salt.
A technician is testing the purity of a DNA sample using a
spectrophotometer. He gets a 260 nm reading of 0.51 au, and a 280
nm reading of 0.65 au. Recalling that the preferred range for the
value is 1.8-2.0, is the purity of the sample acceptable? - answer No
, Referring to the gel below, what is the approximate size of band B? -
answer Approximately 1,400 base pairs
When using an acrylamide gel for protein electrophoresis, it is best
to use a higher percentage of acrylamide to resolve smaller
proteins, while large proteins are best resolved on low-percent gels.
- answer True
Staining with Coomassie Blue - answer To visualize proteins after
PAGE
Adding a molecular weight standard to an empty well - answer To
determine the relative sizes of the samples run on the gel
Adding SDS to the sample buffer and the PAGE running buffer -
answer Adding SDS to the sample buffer and the PAGE running
buffer
Adding loading dye to protein samples - answer To facilitate loading
samples into wells, and to monitoring sample migration during PAGE
Your supervisor asks you to run a PCR reaction with products
ranging in size from 200-400 base pairs. To confirm the reaction
outcome on an agarose gel, which of the following strategies will
give you the best resolution of the PCR products? - answer Use a
higher percentage of agarose and a lower running voltage
In agarose gel electrophoresis, the negative electrode terminal is
located: - answer At the top of the gel (closest to wells)
A technician is given a sample of DNA from a PCR reaction, and a
sample of uncut plasmid. The PCR product contains a gene of
interest that the technician's supervisor wants cloned into the
plasmid. The technician uses software to determine which enzymes
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