Bio 210 Exam – Questions With Accurate Solutions
Where do you dispose of lab materials after you have used them (swabs,
slides, kimwipes, agar plates, etc.)? Right Ans - -morgue
-biohazard waste bucket
What are some ways in which we practice aseptic technique in the lab?
Right Ans - -wear lab coats, eye googles, gloves
- lysol lab table before and after use
-flame loop before gathering specimens
-wash hands before and after lab
-put outerwear in cabinet
-open and close tube lids w/ one hand and same w/ plates
Why do you need to know the calibration of your microscope for each
objective lens? Right Ans - the magnification and distance between each
ocular unit in diff objective lens is different so need to calibrate in order to get
actual size of specimen
Under a microscope, how can you differentiate between a protozoan and a
bacterial cell? Algae and protozoa? Fungi and bacteria? Right Ans - -
Protozoa: bigger, organelles, nucleus, no cell walls
-Bacteria: smaller, no organelles, no nucleus, cell walls w/ peptidoglycan
-Alage: chloroplast that are light harvesting pigments, green b/c chorophyll
pigment, motile via dinoflagellates, cell wall w/ cellulose, multicellular or
unicellular
-Protozoa: unicellular, no cell wall, motile via glidings, fagella, cilia,
psuedopods
-fungi: bigger, organelles, nucleus, cell wall w/ chitin, non-motile, unicellular
or multicellular
-bacteria: some motile, unicellular, smaller, no organelles, no nucleus, cell wall
w/ peptidoglycan
Algae: features + habitat Right Ans - unicellular + multicellular, chloroplast
organelles that are light haversting pigments that can be utilized to carry out
photosynthesis and use sunlight and CO2 to form glucose and O2, some motile
(dinoflagellates), cell walls w/ cellulose
, Habitat: aquatic
Protozoa: features + habitat Right Ans - unicellular, no cell walls, motile via
glidings-pseudopods- flagella- cilia, some can change between trophozoite and
cyst form
Habitat: soil + aquatic
Fungi: features + habitat Right Ans - can be yeast( unicellular) or mold
(multicellular), or dimorphic (both), cell wall w/ chitin, reproduce by either
spores (mold) or yeast (buds), non-motile
Habitat: warm + humid
Bacteria Right Ans - unicellular, cell walls w/ peptidoglycan, reproduce via
binary fission, vast metabolic capacity, some motile, prok
Habitat: everywhere
What are some precautions that you must take when working with BSL-2
organisms? BSL-1 organisms? Right Ans - BSL-1= work on lab bench or
table, ppe worn as needed, have sink available, door that separates lab from
rest of facility
BSL-2= lab closed off while in session, ppe worn, procedures w/ aerosols or
splashes done under biological safety cabinet, wear face shield as needed, eye
wash and sink available, autoclave or any alternative decontamination waste
basket for proper disposal
Protozoa: Disease Right Ans - Disease: giardiasis
Pathogen: giardia
Transmission: ingestion of water w/ pathogen, through contact from person
to person, and food contamination
symptoms: diarrhea, naseau , vomiting, bloating, new intolerance to diary,
fatigue
Bacteria: Disease Right Ans - Disease: cholera
Pathogen: vibrio cholera