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PMCOL 303 Exam Questions And Accurate Answers
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PMCOL 303 Exam Questions AndAccurate Answers
research is about - Answer asking questions and mastering techniques (collect and
analyze data )
all professionals must integrate - Answer info and think critically
a toxicologist's toolkit encompasses - Answer myriad techniques
animals tests --> - Answer - behaviour and in vivo/ex vivo
- KO animals
types of myriad techniques - Answer - flow cytometry
- microscopy
- western blot
- PCR
- high performance liquid chromatography (HPLC)
whole animal - in vivo and ex vivo tests - Answer - help aim the next assessment (hot
plate for sensing pain --> ion channels)
- remove a tissue (neurons, vascular bed, cell population) and perform experiments
flow cytometry measures - Answer a sub cellular fluorescent signal
flow cytometry requires - Answer sourcing of cell from animals
sophisticated flow cytometers read up to - Answer 6 target specific stains at once
flow cytometry fixes cells and - Answer detects fluorescence of individual cells on a
machine
annexin V stains - Answer phosphatidylserine which appear in apoptotic cell
membranes
JC-1 stains - Answer mito membranes and switches color if mito is unhealthy --> green =
unhealthy, red = healthy
confocal microscopy co-localizes - Answer sub cellular targets
how does confocal microscopy work? - Answer - stain two targets in a sample
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