Exam (elaborations)
Cell Biology Lab Final Exam Review ;100% complete newest version guide (verified)
- Course
- Institution
Cell Biology Lab Final Exam Review ;100% complete newest version guide (verified)
[Show more]
Content preview
Cell Biology Lab Final Exam Review- ANS-Can you calculate distances between each RE cut on a plasmid?
- ANS-Can you determine # of fragments if RE cut your plasmid?
- ANS-Can you predict a Western Blot given MW's, Ab's and tagged proteins?
- ANS-Can you recognize format of restriction enzymes if examples provided for you?
- ANS-Can you recognize restriction enzymes you worked with in lab 4 such as EcroR I, Bcl I,
Xba I, etc?
- ANS-Do you know what GAPDH is?
- ANS-Draw predicted gel with following molecular weights: 50kDa, 35kDa, 100kDa, 500kDa.
- ANS-I will not limit myself to only this review when I study for midterm.
- ANS-Review definition and function of DHFR
- ANS-Review what recombinant proteins are and where are they used!
A) 2, 16 - ANS-QQ: RUBISCO is holoenzyme with ____ types of subunits for total of _____
subunits.
A) 2, 16
B) 8, 8
C) small, large
A) 260, 280 - ANS-Nucleic acids absorb maximally at ___ nm while proteins absorb maximally
at ____ nm.
A) 260, 280
B) 280,260
C) 280,260
D) 595, 260
A) 32 ul - ANS-If you need 8ul of 5ug/ul P+1xL sample and want total volume of 40ul, how much
1xL do you need?
A) 32ul
, B) 32
C) 35ul
D) 40ul
A) 3560 ul - ANS-3.56mL is _______ ul?
A) 3560 ul
B) 35.6 ul
C) 356 ul
D) 0.356 ul
A) 8 ul - ANS-5ug/ul of P+L, what volume of it do you need for 40ug?
A) 8 ul
B) 0.125 ul
C) 8
D) 0.125
A) EtBr - ANS-This reagent intercalates in DNA but allows us to visualize bands using UV lamp:
A) EtBr
B) Coomassie
C) Laemmli
D) Water
A) Expasy - ANS-You used this computational resource to calculate MW
A) Expasy
B) Blast
A) Expasy - ANS-You used this computational resource to translate sequences
A) Expasy
B) Blast
A) Parallel - ANS-What type of dilutions were you instructed to use to create standard curve?
A) Parallel
B) Serial
C) We used both
D) None of the above
A) Remove any excess anitbodies from membrane - ANS-Purpose of washes was to:
The benefits of buying summaries with Stuvia:
Guaranteed quality through customer reviews
Stuvia customers have reviewed more than 700,000 summaries. This how you know that you are buying the best documents.
Quick and easy check-out
You can quickly pay through credit card or Stuvia-credit for the summaries. There is no membership needed.
Focus on what matters
Your fellow students write the study notes themselves, which is why the documents are always reliable and up-to-date. This ensures you quickly get to the core!
Frequently asked questions
What do I get when I buy this document?
You get a PDF, available immediately after your purchase. The purchased document is accessible anytime, anywhere and indefinitely through your profile.
Satisfaction guarantee: how does it work?
Our satisfaction guarantee ensures that you always find a study document that suits you well. You fill out a form, and our customer service team takes care of the rest.
Who am I buying these notes from?
Stuvia is a marketplace, so you are not buying this document from us, but from seller stephanvdb04. Stuvia facilitates payment to the seller.
Will I be stuck with a subscription?
No, you only buy these notes for $7.99. You're not tied to anything after your purchase.
Can Stuvia be trusted?
4.6 stars on Google & Trustpilot (+1000 reviews)
75632 documents were sold in the last 30 days
Founded in 2010, the go-to place to buy study notes for 14 years now