CBC Methods and RBC Analytes (L2 W1)
What blood tube do we use for CBC? Any additives?
purple top & EDTA
What blood tube do we use for serum electrolyte panels? Any additives?
red top & none
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What blood tube do we use for clotting profile? Any additives?
bleu top & Na citrate
What blood tube do we use for whole blood chem? Any additives?
green top & heparin
Why is whole blood quicker to read?
do not have to wait for it to clot or centrifuge
What are the three electrograms we see on a CBC?
- CBC use a purple top = whole blood
, leukogram, erythrogram, thrombogram
What does RBC mean on an erythrogram?
erythrocyte concentration per uL of blood
How do we measure the RBC in an erythrogram? Explain
impedance counting
- each RBC interferes with electron flow caused by cathode & anode + creating a
pulse that can be measured
What analytes measure the RBC mass?
Hematocrit (Hct), Hemoglobin (Hgb), and RBC
Hematocrit (Hct) measures mass of RBC in peripheral blood by _____
% of RBC in a certain volume of your blood
Hemoglobin (Hgb) measures mass of RBC in peripheral blood by __________ of
Hgb in RBCs after ____
spectrophotometer (wavelength of light); lysis
hemoglobin measures the ___ of Hbg /. ____ of blood
g of Hgb / dL of blood
Why must the RBC become lysed in order for Hgb to be recorded?
RBC lysis allows Hgb to be released & projects a wavelength that can be
recorded via a spectrophotometer
In general, Hct =
Hgb x 3
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