Red Blood Cell Count
red blood cell count - ANS is the number of red cells in 1 mm^3 of blood
isotonic solution - ANS blood is diluted with an _____ solution to preserve the
blood corpuscles
Gower's diluting fluid - ANS diluting fluid used in RBC count
must be an isotonic solution
must have a high specific gravity
must be easy to prepare
must be cheap
must be a good preservative
must have a buffer action
must not initiate growth of molds - ANS Characteristics of a good RBC diluting
fluid
0.5 mark - ANS aspirate blood up to the ____ mark of the RBC pipette
diluting fluid - ANS where to place the pipette after aspirating 0.5 mark of blood
101 mark - ANS aspirate the diluting fluid up to the _____ mark
1:200 - ANS dilution of RBC pipette
5-6 drops - ANS number of drops to be discarded of the diluted blood from the
pipette
30°-35° - ANS angle of the pipette while charging
LPO - ANS locate the ruled area for RBC counting by focusing the microscope
using the ______
HPO - ANS objective used for actual counting
, five tertiary squares - ANS count the RBCs seen on the _______ of the counting
chamber
1/5 mm^2 - ANS area of the five squares
20 or less - ANS cell difference between the squares of RBC count
4.5-6.0 M/mm^3 - ANS normal values of RBC count in males
4.0-5.5 M/mm^3 - ANS normal values of RBC count in females
3.0-5.0 M/mm^3 - ANS normal values of RBC count in late pregnancy
7.0 M/mm^3 - ANS normal values of RBC count at birth
I. microscopic methods
ii. automated methods
iii. photoelectric methods - ANS Other Methods of RBC count
a. Unopette system
b. Tube dilution - ANS microscopic methods
unopette system - ANS is a convenient method of preparing diluted blood
sample for cell counting
unopette system - ANS it uses a pre measured volume of diluting fluid in a
plastic reservoir
capillary pipette - ANS a specific amount of blood is then collected using a
_______ and mixed with the diluting fluid
tube dilution - ANS uses 0.02 mL of blood plus 4 mL of diluting fluid
a. sysmex E-5000
b. sysmex NE-8000 - ANS automated methods
sysmex E-5000 - ANS analyzes and reports CBC, RBC indices, red blood cell
distribution with (RDW) platelet count, MVP and nucleated cells according to size
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