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BCH5413 Test Questions Solved 100% Correct | Latest Update
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BCH5413 Test Questions Solved 100% Correct| Latest Update
When 454 pyrosequencing was developed, what were the two main advances in
the method that allowed for faster sequences of millions of templates? - Answer -
the ability to determine the sequence without the use of a gel matrix.
-the addition of adapters to the fragmented DNA to allow sequencing of any
DNA fragment (the ligation of adapters to the ends of the DNA fragments
allowed the same primer to be used for all sequencing reactions)
Which of the following statements best describes a heterozygous gene locus?
a. The two chromosomes have different alleles at the locus
b. One of the two chromosomes has a mutation at the locus
c. The two chromosomes have the same alleles at the locus
d. The chromosome is replicated and has a sister chromatid - Answer a. The two
chromosomes have different alleles at the locus
*This is the standard definition of the term heterozygous.
b. One of the two chromosomes has a mutation at the locus
*Heterozygous means the two alleles are different. This case (one with and
one without a mutation) is one example of how the alleles of a gene could be
heterozygous.
, the melting temperature (Tm) of DNA increases with ----- GC content and -----
salt concentration - Answer higher/higher
In the experiment that supports DNA as the genetic material, why does the
mouse live if it is injected with only the dead pathogenic strain? - Answer You
need live bacteria to take up DNA from the dead bacteria (and be
transformed) for the mouse to die.
The Hi-C conformation capture experiment can be used to investigate the
organization of chromatin within the nucleus of a cell under specific cellular
conditions. In this method, the purpose of the crosslinking step is to... - Answer
To join two fragments of DNA that are interacting.
In chromosome structure, the core histones function to form... - Answer The
nucleosome
What type of mutation can be determined using the Western Blot technique? -
Answer If a mutated protein has altered mobility compared to the normal
*The protein would be run on an acrylamide gel according to size and charge, and
then detected with an specific antibody. If a protein had altered mobility it would
be detected.
Gel electrophoresis separates nucleic acids on the basis of... - Answer Size
*Larger sizes have more difficult traveling through the gel matrix an run
slower than smaller fragments.
Which of the following statements regarding the stringency of a Southern Blot is
correct? - Answer Decreasing the ionic strength increases stringency because
less ions in solution make it more difficult for DNA fragments to anneal.
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