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BIOL 2420: Exam #1 Practice Questions and Answers | 100% Pass

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BIOL 2420: Exam #1 Practice Questions and Answers | 100% Pass What are the 6 categories of microorganisms? - Answer️️ -Bacteria, archaea, fungi, protozoa, algae, and helminths. What are the differences between prokaryotes and eukaryotes? - Answer️️ - Prokaryotes: Unicellular, lack a nu...

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  • September 13, 2024
  • 22
  • 2024/2025
  • Exam (elaborations)
  • Questions & answers
  • BIOL 2420
  • BIOL 2420
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©SOPHIABENNETT
EXAM_SOLUTIONS 2024/2025 ACADEMIC YEAR




©SOPHIABENNETT 9/2/24 2024/2025

,©SOPHIABENNETT_2024/2025 EXAM SERIES Monday, September 2, 2024 9:29 AM


BIOL 2420 Lab Practical 1 Questions
and Answers | 100% Pass


What is the single most important way to reduce the spread of unwanted microbes? -

Answer✔️✔️-handwashing


Where and how should used media (culture tubes and plates) be disposed of? -

Answer✔️✔️-directly into bio hazard bin

Where and how should a slide prepared by you (ex: Gram stain) be disposed of? -

Answer✔️✔️-Wrapped in a paper towel and disposed into the biohazard bin

Where should gloves worn by you (after lab, not cleaning up a spill) be disposed of? -

Answer✔️✔️-in the regular trash can

Where should a broken stock slide (ex: 3 forms slide that was observed during lab 3) be

disposed of? - Answer✔️✔️-in the broken glass container

What are the last two things that you always do before leaving the microbiology lab that

will ensure that you do not spread organisms used in the lab out of the lab and will also

ensure that the next class coming into the lab will not be contaminated? - Answer✔️✔️-1.

disinfect lab bench

2. wash hands

Select all required information needed to properly label your cultures when labeling

culture media. - Answer✔️✔️-1. name of medium (TSA, TSB)

2. date of inoculation

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, ©SOPHIABENNETT_2024/2025 EXAM SERIES Monday, September 2, 2024 9:29 AM

3. the inoculum (microbes name)

4. your initials (F, M, L)

liquid media - Answer✔️✔️-media that does not contain agar

solid media - Answer✔️✔️-media that contains the solidifying agent, agar, in it

incinerator - Answer✔️✔️-Equipment used to sterilize the inoculating loops via

incineration

inoculate - Answer✔️✔️-Introduce cells or organisms into a culture medium

inoculum - Answer✔️✔️-Organism or material used for inoculation

incubator - Answer✔️✔️-Equipment used to grow microorganisms in a controlled

environment

Why is it incorrect to label a culture tube cap or the lid of the petri dish? - Answer✔️✔️-1.

Lids can easily fall off or be switched.

2. Plate media is incubated upside down

Identify where you would place the culture in the following scenario:

You inoculated a broth with E. coli in lab and want to grow the culture for 48 hours and

will check on the culture during the next lab period. - Answer✔️✔️-37 degrees C

Identify where you would place the culture in the following scenario:

You inoculated a broth with E. coli in lab but did so at the end of the week. You won't be

back in lab until after the weekend. Therefore, the growth needs to be slowed down as

the incubation time will be longer than 72 hours. - Answer✔️✔️-25 degrees C

Identify where you would place the culture in the following scenario:




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