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CHEM 219 Organic Bio 219 Final questions with answers A+ graded $7.99   Add to cart

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CHEM 219 Organic Bio 219 Final questions with answers A+ graded

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  • CHEM 219 Organic Bio 219
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  • CHEM 219 Organic Bio 219

What two things need to be oxidized for fluorescence to occur? - - aldehyde -flavin mononucleotide Luciferase - -enzyme that catalyzes the light emitting reaction -has alpha and beta subunits Lux I - -encodes for auto-inducer Lux R - -encodes for transcriptional activator that binds the auto ...

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  • September 15, 2024
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  • CHEM 219 Organic Bio 219
  • CHEM 219 Organic Bio 219
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CHEM 219 Organic Bio 219 Final

What two things need to be oxidized for fluorescence to occur? - ✔✔-
aldehyde
-flavin mononucleotide


Luciferase - ✔✔-enzyme that catalyzes the light emitting reaction
-has alpha and beta subunits


Lux I - ✔✔-encodes for auto-inducer


Lux R - ✔✔-encodes for transcriptional activator that binds the auto inducer
-transcribed oppositely all other subunits


Two aspects of gram-negative bacteria - ✔✔-external LPS layer
(lipopolysaccharide)
-single layer of peptidoglycan based cell wall


Operon - ✔✔-genetic regulatory system in which genes coding for
functionally related proteins are clustered along the DNA


Beneficial aspects of bioluminescence - ✔✔-communication
-attracting prey
-hiding from predators
-attracting a mate

, -reliable food source for bacteria (glucose + amino acids)


Two types of organisms that use bioluminescence - ✔✔-pinecone fish
-hawaiian bobtail squid


Quorum Sensing - ✔✔-regulation of gene expression in response to
changes in cell-population density


Auto-induction - ✔✔-bacteria release a small metabolic product, N-Acyl
homoserine lactone, which diffuses across membrane into extracellular
environment


Two Part Purpose of class - ✔✔-create a complete Aliivibrio fischeri
genomic library
-create a restriction map of lux containing plasmid


Aliivibrio fischeri - ✔✔-gram negative
-rod shaped
-chromosomal DNA contains lux operon




Lux C - ✔✔-encodes for the acyl-reductase enzyme


Lux D - ✔✔-encodes for the acyl-transferase enzyme

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