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CMB2001 L01 Questions and Answer | Latest Update 2024/2025

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Consensus sequences are described with IUPAC ambiguous nucleotide codes. W is A or T S is G or C R is G or A Y is T or C What is the span of the core/basal region of the eukaryotic promoter? ~~> -30 to +30nt What elements are in the core region of the eukaryotic promoter? ~~> TATA...

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  • September 15, 2024
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  • 2024/2025
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1 | P a g e | © copyright 2024/2025 | Grade A+




CMB2001 L01 Questions and
Answer | Latest Update 2024/2025
Consensus sequences are described with IUPAC ambiguous nucleotide

codes.


W is A or T


S is G or C


R is G or A


Y is T or C




What is the span of the core/basal region of the eukaryotic promoter?


✓ ~~> -30 to +30nt




What elements are in the core region of the eukaryotic promoter?


✓ ~~> TATA box

Inr (initiator)


DPE (downstream promoter element)


BRE (TFIIB recognition element)




Master01 | September, 2024/2025 | Latest update

, 2 | P a g e | © copyright 2024/2025 | Grade A+


Describe the TATA box consensus sequence


✓ ~~> -31 to -26nt, TATAWAWR




Describe the Inr sequence


✓ ~~> -2 to +4nt, YYANWYY with an A at +1




What is observed in core promoter CpG islands without obvious TATA and Inr

sequences?


✓ ~~> Lower transcription rate, initiation at multiple start sites




What is the difference between a distal and proximal regulatory promoter

element?


✓ ~~> Proximal = within 1kb of the start site

Distal = 1kb or more away from the start site




How do you identify promoter elements via sequence comparison?


✓ ~~> Analyse base frequency within many genes to compare and

identify a consensus sequence




How do you identify promoter elements via reporter analysis?



Master01 | September, 2024/2025 | Latest update

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