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BIOD 171 Module 4 Quiz with Verified Answers |Latest 2024/2025

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What are the requirements of a fastidious microbe? A fastidious microbe is an organism with complex growth requirements and will not grow without then. Enriched medias thus contain these specific and essential nutrients required for the growth of a particular subset of microorganisms. What is the name of the process of spreading a bacterial culture onto a petri dish? Plating. Plating microbes can be done using a sterile loop, a sterile swab, or a sterilized wire loop. Each device is simply a means of spreading the bacteria, most commonly in a simple back-and-forth motion, across the plate. What is the primary advantage of plating a bacterial culture as opposed to having it grow in solution? The primary advantage of plating a bacterial sample onto agar is that cells are held in place. Unlike in a nutrient broth where bacterial cells can multiply but are free to move around in solution, bacteria plated onto agar are fixed in such a way as to support the formation and visualization of colonies. What is the purpose of the quadrant streak approach? The purpose of this method is to generate an individual colony so that a single (pure) bacterial sample can be picked from the plate.

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biod 171 module 4 quiz
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biod 171 module 4 quiz with verified answers

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BIOD 171 Module 4 Quiz with Verified
Answers.

What are the requirements of a fastidious microbe?
_;- _;- _;- A fastidious _;- _;- _;- _;- _;-_;- _;- _;- _;-

microbe is an organism with complex growth requirements and will not
_;- _;- _;- _;- _;- _;- _;- _;- _;- _;- _;-

grow without then. Enriched medias thus contain these specific and
_;- _;- _;- _;- _;- _;- _;- _;- _;- _;-

essential nutrients required for the growth of a particular subset of
_;- _;- _;- _;- _;- _;- _;- _;- _;- _;- _;-

microorganisms.

What is the name of the process of spreading a bacterial culture onto a
_;- _;- _;- _;- _;- _;- _;- _;- _;- _;- _;- _;- _;- _;-

petri dish?
_;- Plating. Plating microbes can be done using a sterile
_;-_;- _;- _;- _;- _;- _;- _;- _;- _;- _;- _;-

loop, a sterile swab, or a sterilized wire loop. Each device is simply a
_;- _;- _;- _;- _;- _;- _;- _;- _;- _;- _;- _;- _;- _;-

means of spreading the bacteria, most commonly in a simple back-and-
_;- _;- _;- _;- _;- _;- _;- _;- _;- _;-

forth motion, across the plate.
_;- _;- _;- _;-

What is the primary advantage of plating a bacterial culture as opposed
_;- _;- _;- _;- _;- _;- _;- _;- _;- _;- _;- _;-

to having it grow in solution?
_;- The primary advantage of plating a
_;- _;- _;- _;- _;-_;- _;- _;- _;- _;- _;- _;- _;-

bacterial sample onto agar is that cells are held in place. Unlike in a
_;- _;- _;- _;- _;- _;- _;- _;- _;- _;- _;- _;- _;- _;-

nutrient broth where bacterial cells can multiply but are free to move
_;- _;- _;- _;- _;- _;- _;- _;- _;- _;- _;- _;-

around in solution, bacteria plated onto agar are fixed in such a way as
_;- _;- _;- _;- _;- _;- _;- _;- _;- _;- _;- _;- _;- _;-

to support the formation and visualization of colonies.
_;- _;- _;- _;- _;- _;- _;-

What is the purpose of the quadrant streak approach?
_;- _;- _;- The purpose _;- _;- _;- _;- _;- _;-_;- _;- _;- _;-

of this method is to generate an individual colony so that a single (pure)
_;- _;- _;- _;- _;- _;- _;- _;- _;- _;- _;- _;- _;- _;-

bacterial sample can be picked from the plate.
_;- _;- _;- _;- _;- _;- _;-

,What are the requirements of a fastidious microbe?
_;- _;- A fastidious
_;- _;- _;- _;- _;- _;-_;- _;- _;- _;-

microbe is an organism with complex growth requirements such that if
_;- _;- _;- _;- _;- _;- _;- _;- _;- _;- _;-

absent it will not grow. Enriched medias thus contain these specific and
_;- _;- _;- _;- _;- _;- _;- _;- _;- _;- _;- _;-

essential nutrients required for the growth of a particular subset of
_;- _;- _;- _;- _;- _;- _;- _;- _;- _;- _;-

microorganisms.

What is agar? _;-In its simplest form agar is liquid growth media that
_;- _;-_;- _;- _;- _;- _;- _;- _;- _;- _;- _;- _;- _;-

has had a hardening agent added to it. Agar is created when a
_;- _;- _;- _;- _;- _;- _;- _;- _;- _;- _;- _;- _;-

polysaccharide derived from seaweed (algae) extract is added to growth
_;- _;- _;- _;- _;- _;- _;- _;- _;- _;-

media. Agar is used to create a solid, smooth surface on which microbes
_;- _;- _;- _;- _;- _;- _;- _;- _;- _;- _;- _;- _;-

can grow.
_;-

LB agar is classified as a non-selective, differential media
_;- _;- _;- _;- _;- _;- _;- _;-

True
False _;-_;- _;- False

LB agar is non-selective and non-differential
_;- _;- _;- _;- _;-

What are the three forms of hemolysis found on blood agar plates?
_;- _;- _;- _;- _;- _;- _;- _;- _;- _;- _;- _;-_;- _;-

Alpha hemolysis, beta hemolysis, and gamma hemolysis
_;- _;- _;- _;- _;- _;-

Alpha hemolysis _;- Presents with greenish-brown color on blood agar _;-_;- _;- _;- _;- _;- _;- _;- _;- _;-

due to the partial lysis of red blood cells
_;- _;- _;- _;- _;- _;- _;- _;-

, Beta hemolysis
_;- Clear zones on blood agar indicate beta hemolysis, _;-_;- _;- _;- _;- _;- _;- _;- _;- _;- _;-

which is the complete lysis of red blood cells
_;- _;- _;- _;- _;- _;- _;- _;-

Gamma hemolysis Presents a white/tan on blood agar due to the
_;- _;-_;- _;- _;- _;- _;- _;- _;- _;- _;- _;- _;-

lack of hemolytic activity.
_;- _;- _;-

What is the primary purpose of Columbia CNA agar?
_;- _;- _;- As it _;- _;- _;- _;- _;- _;-_;- _;- _;- _;-

suppresses the growth of Gram-negative bacteria CNA agar is, therefore,
_;- _;- _;- _;- _;- _;- _;- _;- _;- _;-

used for isolation of Gram-positive microbes.
_;- _;- _;- _;- _;-

Name the type of plate derived from BAP that contains lysed red blood
_;- _;- _;- _;- _;- _;- _;- _;- _;- _;- _;- _;- _;-

cells Lysed red blood cells (RBCs) are a primary component in the
_;-_;- _;- _;- _;- _;- _;- _;- _;- _;- _;- _;- _;- _;-

formulation of Chocolate agar. _;- _;- _;-

Define the selective and differential abilities of a MacConkey agar plate
_;- _;- _;- _;- _;- _;- _;- _;- _;- _;- _;-_;-

MacConkey agar is selective in that only Gram-negative microbes
_;- _;- _;- _;- _;- _;- _;- _;- _;- _;-

will grow on the agar -Gram-positive microbes simply do not grow.
_;- _;- _;- _;- _;- _;- _;- _;- _;- _;- _;-

MacConkey agar is also considered a differential media as it
_;- _;- _;- _;- _;- _;- _;- _;- _;- _;-

distinguishes between microbes capable of fermenting lactose (red
_;- _;- _;- _;- _;- _;- _;- _;-

colonies) and those that are non-fermenters (white/tan colonies).
_;- _;- _;- _;- _;- _;- _;-

What colors would you expect to see on an EMB plate containing E.
_;- _;- _;- _;- _;- _;- _;- _;- _;- _;- _;- _;- _;-

coli? The EMB plate itself is red in color while in the presence of E.
_;-_;- _;- _;- _;- _;- _;- _;- _;- _;- _;- _;- _;- _;- _;- _;- _;-

coli, the growing colonies will take on a distinctive metallic green sheen.
_;- _;- _;- _;- _;- _;- _;- _;- _;- _;- _;-

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