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MLS ASCP EXAM REVIEW QUESTIONS AND ANSWERS GRADED A+
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MLS ASCP EXAM REVIEW QUESTIONS ANDANSWERS GRADED A+
What tests are preformed on donor blood?
ABO, Rh (anti-D), Antibody screen (Rh, Kell, Kidd, Duffy), serologic tests (syphillis), viral
diseases (HCY-RNA, HBsAg, Anti-HBc)
One unit of RBCs increases hgb and hct by how much?
Hgb: 1g
Hct: 3%
For leukoreduced RBCs the final WBC count must be?
<5x10^6
Dyglycerated cells are protected from low temps with what concentration of glycerol
agent?
40%
One unit of platelets increases platelet count by
5,000-10,000
One unit of apheresed platelets should increase the platelet count _________.
20,000-60,000
what do irradiated blood components do?
prevent GVHD by innactivating T-cells
Plasma should be stored at
-18C
RBCs should be stored at
1-6C
platelets should be stored at
22C
,Group O has the greatest amount of what antigen?
H
Group A1B has the least amount of what antigen?
H
Type O blood has __ antigens
Neither A nor B
Type AB has what antigens?
A and B antigens
Type A blood has what antigens?
A antigens
Type B blood has what antigens?
B antigens
Type O blood has what antibodies?
anti-A and anti-B antibodies
Type AB blood has what antibodies?
no antibodies
Type A blood has what antibodies?
anti-B antibodies
Type B blood has what antibodies?
anti-A antibodies
Type O blood can receive
O only
Type O blood can donate to
A, B, AB, O (universal donor)
Type AB blood can receive
A, B, AB, O (universal recipient)
Type AB blood can donate to
,AB only
Type A blood can receive
types A and O
Type A blood can donate to
Type A and Type AB
Type B blood can donate to
Type B and Type AB
Type B blood can receive
Type B and Type O
unknown RBCs + anti-A = agglutination indicating the cells DO have the the antigen in
the antisera. This is an example of?
Forward blood typing
unknown plasma + A1 cells = agglutination, indicating the serum HAS the antibody to the
antigen on the red cells. This is an example of?
reverse blood typing
unknown plasma + B cells = agglutination, indicating the serum HAS the antibody to the
antigen on the red cells. What is the patient's reverse type?
negative
unknown RBCs + anti-B = agglutination indicating the cells DO have the the antigen in
the antisera. What is the patient's forward type?
B
A patient exhibits agglutination in the tube containing anti-A + RBCs and in the tube
containing plasma + B cells. What is the patients blood type?
A-
What are clinically significant antibodies?
IgG antibodies including: Kell, Kidd, Duffy, ABO, Rh, (S,s,U), and Vel
91% of people are negative for what IgG antibody?
Kell (K)
What IgM antibody is negative on infant cells but positive on adult?
, Anti-I
What IgM antibody is NOT present on chord cells?
LeA and LeB
Is IAT in-vivo or in-vitro?
in vitro
Is DAT in-vivo or in-vitro?
in vivo
True or false: IAT detects antibodies in plasma
true
True or false: False positives in IATs are caused by inadequate washing, under
centrifugation, and delay in adding antiglobulin reagent
false
false NEGATIVES are caused by those things
True or False: a positive IAT indicates the blood is NOT compatible.
true
Adding LISS to the serum and red cells enhances:
allows for stronger reactions and speeds up test.
Adding enzymes (bromelin, ficin, papin, and trypsin) increases reactivity of what
antibodies?
Rh, Kidd, and Lewis
Adding enzymes (bromelin, ficin, papin, and trypsin) destroys what antibodies?
M, N, S, Fya & Fyb
PEG increases the strength of agglutination reactions by:
removing water
Antiglobulin in DAT reacts with what?
IgG antibodies/complement
What sample type is preferred for DAT's because it prevents complement activation?
EDTA
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