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BIO 1404 Practical 1 Lab 1; Questions and Answers
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BIO 1404 Practical 1 Lab 1; Questions and Answers
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BIO 1404 Practical 1 Lab 1; Questions and AnswersDo free amino acids have peptide bonds? Correct Ans-No because a peptide bond is a
bond between two amino acids.
What do you conclude about the solubility of lipids in polar solvents such as water compared
to nonpolar solvents such as acetone? Correct Ans-In the distilled water, the lipids did not
mix well with the distilled wat which was why there was a semi-solid of lipids that stay on
top. In the acetone solution, there was no clear distinction between the lipds and solution
because it was clear.
What happened when the emulsifier was added? Correct Ans-Bubbles formed and rose to
the top of the test tube, there was also a foggy white solution left over
Which one of the solutions is the positive control, and which one of the solutions is the
negative control for the Sudan IV reaction? Correct Ans-the Positive coontrol was the salad
oil and lipid solution. The negative control was the distilled water
Compare tubes 1 and 3 in the second experiment. What is the distribution of the dye with
respect to the separated water and oil? Correct Ans-In tube 3, the distilled water, the
, BIO 1404 Practical 1 Lab 1; Questions and Answers
solution was almost fully clear while in tube 1, the salad oil produce a more toned pink after
the 5 drops of Sudan iV since it was an oil solution.
Which one of the solutions is the positive control, and which one of the solutions is the
negative control for the Dische reaction? Correct Ans-the positve control are the solution
with DNA solution, which the negative controls are the solutions without DNA solution.
How does the color compare between tubes 1 and 2? Why? Correct Ans-In tube 1, the blue
color it produce was a cloudy blue, while the blue produce in Tube 2 was a more clear light
blue. The reason for this is because in tube 1, the DNA solution was more concentraled than
the DNA solution in tube 2, because it was diluted a bit by the distilled water
Which one of the solutions is the positive control, and which one of the solutions is the
negative control for the Benedict's reaction? Benedict's test contains REAGENT CUPRIC
COPPER ION Correct Ans-The positve control are the solutions with monosaccharides with
glucose or fructose which changes the color of the solution to either green or reddish orange.
The negative control was the solutions that remained blue which was distilled water, potato
juice, and 1% starch solution since it doesnt reduce sugars.
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