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MCB 301 Exam Questions and Answers | 100% Pass

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MCB 301 Exam Questions and Answers | 100% Pass Pure Culture - Answer️️ -Population of cells consisting of one type of bacterium derived from a single cell. a population of cells that are all clones and identical to each other that originally came from a single cell. if you have a colony gro...

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  • September 18, 2024
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  • MCB 301
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MCB 301 Exam Questions and Answers
| 100% Pass

Pure Culture - Answer✔️✔️-Population of cells consisting of one type of bacterium

derived from a single cell. a population of cells that are all clones and identical to each

other that originally came from a single cell. if you have a colony growing on a solid

media and then bacterial cells divide by binary fission you'll have a million cells.


Why do you want a pure culture - Answer✔️✔️-To study a bacterial cell's metabolism or

morphological characteristics

How do you produce a pure culture? - Answer✔️✔️-Requirements: 1. growth media for

cell nutrition 2. sterilization to remove all contaminants 3. aseptic technique to prevent

contamination of the yourself, the materials and specimen

The most common method is streak plating and you may have to do it more than once.

Streak Plates - Answer✔️✔️-You are diluting as you are streaking. you are picking up

bacteria and putting less and less in each quadrant

Mixed Cultures - Answer✔️✔️-This is when you have more than one type of organism on

each plate. each organism might produce a characteristic colony type.

How do you produce a pure culture - Answer✔️✔️-1. streak plating which is the most

common method.

2. spread plating

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Spread Plating - Answer✔️✔️-You dilute the cells before you plate them. Then you put

your inoculum on the plate and use the hockey stick to spread the inoculum around.

Luminescent Bacteria - Answer✔️✔️-Gram negative

motile

polar

flagella

they may live in sea water and have high osmolarity

Bioluminescence reaction - Answer✔️✔️-RCHO + FMNH2 + O2 --> RCOOH + FMN +

H20+ hb

Qurom sensing - Answer✔️✔️-allows a single cell to sense the population density.

Why stain bacteria - Answer✔️✔️-Bacterial cells are mostly water and are transparent to

view. Bacteriological stains are dyes that make them more visible by increasing the

contrast.

Simple Stains - Answer✔️✔️-All cells stain alike and usually requires only one dye. the

types are positive and negative.

Positive Simple Stains - Answer✔️✔️-Basic stains are cationic. They bind to the

negatively charged cell surface. the bacterial cells are dark and colored against a light

background.

negative simple stains - Answer✔️✔️-acidic stains are anionic. the bacterial cells are

negatively charged and they will repel the dye. they are light against a dark background.

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Differential Stain - Answer✔️✔️-Discriminates between different cell types. it usually

requires more than one dye.

The Gram Stain - Answer✔️✔️-The Gram Stain is a type of differential stain and it uses

the two dyes crystal violet and safranin.

Gram Stain Procedure - Answer✔️✔️-1. Heat Fix the cells to the slide

2. do the primary stain with crystal violet

3. fix the stain with iodine

3. decolorize with ethanol. the gram positive will hold onto the stain more strongly and

the gram negative don't hold onto the stain

4. stain with safranin which is the counter stain. the gram positive will stain purple and

the gram negative will be pink.

gram positive bacteria - Answer✔️✔️-cells stain purple based on cell wall structure.

gram negative - Answer✔️✔️-cells stain pink based on cell wall structure.

Gram positive cell wall - Answer✔️✔️-- there is a thick layer of peptidoglycan.

-it is composed of alternating N-acetylmuramic acid and n- acetylglucosamine residues.

-chains of peptidoglycan cross linked by peptides provide strength

-suface proteins NAM is linked to Teichoic acid and lipoteichoic acid is inserted in the

cytoplasmic membrane

-it prevents osmotic lysis.

-permeable to nutrients

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