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BIOS 280 Lab Quiz 2; Questions and Answers 100% Pass $13.99   Add to cart

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BIOS 280 Lab Quiz 2; Questions and Answers 100% Pass

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  • Course
  • BIOS 280
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  • BIOS 280

BIOS 280 Lab Quiz 2; Questions and Answers 100% Pass

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  • September 18, 2024
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  • Exam (elaborations)
  • Questions & answers
  • BIOS 280
  • BIOS 280
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BIOS 280 Lab Quiz 2; Questions and Answers 100% Pass


the first step in the gram-stain process is to apply ____ stain to a heat-fixed bacterial smear;

the stain is then rinsed off with _____ Correct Ans-crystal violet


water




the second step in the gram-stain process is that the ____ solution is added to the slide; this

acts as a ____, which binds the crystal violet dye to the peptidoglycan layer in the cell wall

Correct Ans-iodine


mordant




the third step in the gram-stain process is that the bacterial smear is ____ with alcohol; any

dye not firmly fixed into the cell wall will wash out; then safranin, a ____, is applied to the

smear Correct Ans-decolorized


counterstain




gram-positive organisms will retain the crystal violet in their thick cell walls with the help of

the mordant and appear ____ Correct Ans-purple

,BIOS 280 Lab Quiz 2; Questions and Answers 100% Pass
the alcohol removes the purple stain from gram-negative organisms, and the colorless cells

take up the safranin counterstain, which appear ____ Correct Ans-pink




____-fixing prevents the bacteria from washing off the slide Correct Ans-heat




the first step in heat-fixing the bacteria is to place 1-2 loopfuls of distilled water on a slide; be

sure to use an ____ loop; aseptically transfer a small amount of bacterial culture from a plate;

mix the culture with the distilled water on a slide; spread the liquid evenly over the slide

Correct Ans-incinerated




the second step in heat-fixing the bacteria is to allow the slide to ____-dry; it is dry when

there is no visible moisture or wetness on the slide Correct Ans-air




the third step in heat-fixing the bacteria is to heat-fix the slide by either leaving the slide on

the slide warmer for ____ minutes, or holding the slide (with a clothespin) against the outside

of a turned-on incinerator for ____ minutes Correct Ans-10


1-1.5




after the slide is heat-fixed, the slide is ready for ____ Correct Ans-staining

, BIOS 280 Lab Quiz 2; Questions and Answers 100% Pass


the first step to perform a gram-stain is to place the slide on the staining rack over the sink;

you may use a clothespin to hold the slide; add 2-3 drops of ____ stain to the slide for about

30 seconds Correct Ans-crystal violet




the second step to perform a gram-stain is to rinse the slide with a small amount of distilled

____ Correct Ans-water




the third step to perform a gram-stain is to add 2-3 drops of Gram's ____ for 30 seconds; rinse

with distilled water Correct Ans-iodine




the fifth step to perform a gram-stain is to hold the slide at a 45 degree angle over the sink;

rinse with ____ until no more purple dye runs off; rinse with distilled water Correct Ans-

alcohol




the seventh step to perform a gram-stain is to add 2-3 drops of ____ for 30 seconds; rinse

with distilled water; carefully (gentle) blot the slide dry with ____ paper Correct Ans-

safranin


bibulous

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