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BCHM 270 Quizzes Solved 100% Correct |Verified Solutions
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BCHM 270 Quizzes Solved 100%Correct |Verified Solutions
A restriction enzyme can be used for which techniques?
a) PCR and cDNA microarray
b) ELISA and qPCR
c) southern blots and cloning
d) northern blots and sds page
e) western blots and enzyme assays - Answer c) southern blots and cloning
the mutation most likely to cause damage to protein function in this list is
a) a mutation in the third position of Thr's ACA codon
b) the mutation of ATG to AGG in the first codon
c) a frameshift mutation near the end of the protein sequence
d) a mutation of CUC to AUC (Leu to Ile) in the middle of the protein - Answer b) the
mutation of ATG to AGG in the first codon
a molecular biologist has digested DNA with Agel, which cuts sites 5'-A/CCGGT-3' and
the reverse compliment sequence 3'-TGGCC/A-5'. which restriction enzyme produces
compatible sticky ends so they can be combined with DNA ligase for cloning?
a) SmaI 5'-CCC/GGG-3'
b) KasI 5'-G/GCGCC-3'
, c) AvaI 5'-C/CCGGG-3'
d_ EaeI 5'-T/GGCCA-3' - Answer c) AvaI 5'-C/CCGGG-3'
which of the following are true for amino acids that have nonpolar side chains?
a) they do not promote hydrophobic interactions
b) they can participate in ionic bonds
c) they tend to cluster on the surface of proteins
d) they do not bind or release protons
e) they can participate in hydrogen bonds - Answer d) they do not bind or
release protons
which of the following correctly describes a peptide bond?
a) the bond is generally in the cis conformation
b) there is free rotation around the bond
c) the bond has a typical single bond character
d) the bond is unstable at 100C in the presence of a strong acid or base
e) the bond is uncharged and nonpolar - Answer e) the bond is unstable at 110C in the
presence of a strong acid or base
which of the following is an example of a motif?
a) the 3D fold of myoglobin
b) the tetrameric structure of hemoglobin
c) the Gly-X-Y sequence of collagen
d) the beta barrel
e) a beta sheet - Answer d) a beta barrel
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