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bioc LAT2 Latest Questions and Answers Rated 100% Correct
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Select the BEST answer. The percentage oxyhaemoglobin in a haemoglobin solution can be calculated by: using the ratio of absorbances of oxy- and deoxyhaemoglobin at wavelengths where the biggest difference in absorbance readings are observed between the two forms. adding a live yeast suspe...

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  • September 21, 2024
  • 22
  • 2024/2025
  • Exam (elaborations)
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  • bioc lat2 latest questions and answers rated 100
  • the percentage oxyhaemoglobin in a haemoglobin sol

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1 | P a g e | © copyright 2024/2025 | Grade A+




bioc LAT2 Latest Questions and
Answers Rated 100% Correct
Select the BEST answer.

The percentage oxyhaemoglobin in a haemoglobin solution can be

calculated by:




using the ratio of absorbances of oxy- and deoxyhaemoglobin at

wavelengths where the biggest difference in absorbance readings are

observed between the two forms.




adding a live yeast suspension to an oxyhaemoglobin solution and observe

the change in absorbance by spectrophotometry.




measuring the absorbance of an oxyhaemoglobin solution using the

wavelength that corresponds to its absorption maxima and then apply the

Beer-Lambert formula.




measuring the absorbance of a haemoglobin solition at 560 nm, which is

deoxyhaemoglobin's absorption maxima and the wavelenth where

oxyhaemoglobin absorb light the weakest.




Master01 | September, 2024/2025 | Latest update

, 1 | P a g e | © copyright 2024/2025 | Grade A+


✓ <~~> using the ratio of absorbances of oxy- and deoxyhaemoglobin

at wavelengths where the biggest difference in absorbance readings

are observed between the two forms.




Absorbance may be defined as:




the ratio of lightpath length to incident light




the log of the ratio of incident light to transmitted light




the ratio of concentration to transmitted light




the ratio of molar absorbance coefficient to lightpath length


✓ <~~> the log of the ratio of incident light to transmitted light




Beer's Law implies that:




the concentration of a solution is dependent on lightpath length




the distance light travels through a solution is independent of concentration




Master01 | September, 2024/2025 | Latest update

, 1 | P a g e | © copyright 2024/2025 | Grade A+




the molar absorbance coefficient is dependent on the concentration of the

absorbing solute




the absorption of light increases with increasing solute concentration


✓ <~~> the absorption of light increases with increasing solute

concentration




When haemoglobin in oxygenated in the lungs it is most likely to:


✓ <~~>




A 3 mL cuvette was filled with 0.5 mL of solution Y and 2.5 mL of water and its

absorbance at 340 nm was 0.394.

The molar absorbance coefficient of solution Y is 31,000 L/mol/cm. The

pathlength is 1 cm.

What is the concentration of the diluted solution Y?




Express your answer in μM and with two decimal points.


✓ <~~> 12.71




Master01 | September, 2024/2025 | Latest update

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