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BIOCHEM Lab Final SHSU Exam Questions and Complete Solutions Graded A+
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BIOCHEM Lab Final SHSU Exam Questions and Complete Solutions Graded A+
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BIOCHEM Lab FinalSHSU Exam
Questions and
Complete Solutions
Graded A+
Denning [Date] [Course title]
,Micropippette - Answer: A pipette is a laboratory tool commonly used in chemistry, biology and
medicine to transport a measured volume of liquid, often as a media dispenser.
Resting-micropipette - Answer: position for when not in use or with liquid drawn
First stop on Micropipette - Answer: Load the sample
Draws indicated volume into the pipette tip
Second stop on Micropipette - Answer: Deliver the sample, completely discharging all of the liquid
Bronsted-Lowry acid - Answer: proton donor (H+)
Bronsted-Lowry base - Answer: proton acceptor
Lewis base - Answer: an atom, ion, or molecule that donates an electron pair to form a covalent bond
Lewis acid - Answer: an atom, ion, or molecule that accepts an electron pair to form a covalent bond
Henderson-Hasselbalch equation - Answer: pH = pKa + log [A-]/[HA]
conjugate base/acid
Find hydronium concentration - Answer: 10e-ph
Find pH - Answer: -log[H+]
Find pKa given Ka - Answer: -logKa
, Find Ka given pKa - Answer: 10e-pka
find pOH given [OH-] - Answer: -log[OH-]
Find [OH-] from pOH - Answer: 10e-pOH
When should you not use the henderson-hasselbach equation ?
When should you use the equation . - Answer: Henderson-Hasselbach should NOT be used for STRONG
acids.
It SHOULD be used for weak acids/base (buffer solutions)
Buffer - Answer: A solution that minimizes changes in pH when extraneous acids or bases are added to
the solution.
Tris buffer (tris hydroxymethyl aminomethane)
What is this used for in Biochemistry Lab?
What two form is it available in ? - Answer: Tris Buffer used for DNA preparations, Protein Experiments
-Exists as Tris Free-base and Tris-HCL
Tris is a weak acid with a pka of 8.075.
What does this information infer about Tris and how can we use it. - Answer: We can infer that Tris
buffer range is between 6-8.
This is ideal buffer range for biological application, with physiological pH being around 7.4
How should you prepare tris buffer ? - Answer: Dissolving mass free tris in water and adjust to desired
pH using HCL
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