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GENETICS TEST 3 DR. SEIBENHENER QUESTIONS AND ANSWERS
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GENETICS TEST 3 DR. SEIBENHENER QUESTIONS AND ANSWERS
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Name: Score:111 Multiple choice questions
Definition 1 of 111
5'- GGCCAATCT- 3'
consensus sequence that occurs upstream by 60-100 bases to the initial transcription site;
typically required for inducible genes to be produced in sufficient amounts
Promoter
Histone tails
Gc box
CAAT box
Definition 2 of 111
+1
AUG codon codes for start
start site of translation
facultative heterochromatin
tata box
start site of transcription
,Definition 3 of 111
1. snRNP U1 binds to 5'
2. U2 binds to BP5 (branch point)
3. Trimer of U 4, 5, 6 recruited to 5' splice site
a. U= snRNPs
4. U1 and 4 dissociate from hnRNA leaving 5 and 6 bound
5. U2 and 6 associate, start to see curvature as 6 and 2 want to come together
6. Lariat (loop) of hnRNA is formed from the intron
7. 5' splice site is cleaved and U5 binds to 3'
8. 3' splice site cleaved and U5 ligates exons together via ATP hydrolysis
9. snRNPs released along with spliced introns, can be recycled and used again
step 0. Pre-Recognition (DNA access)
Spliceosome assembly (happens in order)
Histone acetyl transferase (HAT)
Histone deacetylase (HDAC)
Definition 4 of 111
1. Constitutive
2. Facultative
2 types of chromatin
models to explain association of DNA and proteins
2 types of heterochromatin
5 types of genes
Definition 5 of 111
mitotic spindle arranges chromosomes at cell's equator
Interphase
Telophase
Metaphase
Anaphase
,Definition 6 of 111
extends from the promoter to the terminator
On the DNA:
*regulatory sequence: site for the binding of regulatory proteins
1. promoter: signals beginning of transcription
2. transcribed region: part of this region contains the information that specifies an amino acid
sequence
3. terminator: signals the end of transcription
is transcribed to mRNA
Klinefelter's syndrome (XXY)
step 4. termination
how does the histone H1 work?
the transcriptional unit
Definition 7 of 111
-set of several similar genes, formed by duplication of a single original gene, and generally
with similar biochemical functions
-most often located in similar regions of the chromosome
-most often used or synthesized at different times
Nucleosome (10 nm fiber) and acetylation
step 4. termination
Spliceosome discovery
multigene families
, Definition 8 of 111
process by which a portion of a chromosome is duplicated in an additional copy of a gene
-results in a copy of the original gene called a paralog gene
-either of the 2 genes may mutate and change the original function of the gene
-usually occurs due to an error during meiosis
duplicated genes
multigene families
repeated genes
5 types of genes
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