Micro Bio Lab Exam 1 with correct
Answers
father of hand hygiene - 1846 - Correct Answers -Ignaz Semmelweis
Ignaz Semmelweis, a Hungarian doctor working in Vienna General Hospital, is known
as the father of hand hygiene. In 1846, he noticed that the women giving birth in the
medical student/doctor-run maternity ward in his hospital were much more likely to
develop a fever and die compared to the women giving birth in the adjacent midwife-run
maternity ward. He decided to investigate, seeking differences between the two wards.
He noticed that doctors and medical students often visited the maternity ward directly
after performing an autopsy. Based on this observation, he developed a theory that
those performing autopsies got 'cadaverous particles' on their hands, which they then
carried from the autopsy room into the maternity ward. Midwives did not conduct
surgery or autopsies, so they were not exposed to these particles.
As a result, Semmelweis imposed a new rule mandating handwashing with chlorine for
doctors. The rates of death in his maternity ward fell dramatically. This was the first
proof that cleansing hands could prevent infection.
At a time when most people believed that infections were caused by foul odors called
miasmas, ----- implemented handwashing and other hygiene practices in the war
hospital in which she worked. While the target of these practices was to fight the
miasmas, her handwashing practices achieved a reduction in infections. - Correct
Answers -Florence Nightingale
Sadly, the hand hygiene practices promoted by Semmelweis and Nightingale were not
widely adopted. In general, handwashing promotion stood still for over a century. It was
not until the 1980s, when a string of foodborne outbreaks and healthcare-associated
infections led to public concern that the United States Centers for Disease Control and
Prevention identified hand hygiene as an important way to prevent the spread of
infection. In doing so, they heralded the first nationally endorsed hand hygiene
guidelines, and many more have followed.
How do you wash your hands? - Correct Answers -First wet hands and arms, apply
soap, turn off water and scrub for about 20 seconds (sing happy birthday twice) under
,nails and back of hands, use running water to rinse, and then dry either with paper towel
or air dry
What kind of microscope do we use in class? - Correct Answers -binocular compound
light microscope
What does binocular mean? - Correct Answers -it is used to describe microscopes with
two lenses
the compound microscope is designed for the examination of? - Correct Answers -
transparent specimens
scanning objective - Correct Answers -4x magnification
medium power objective - Correct Answers -magnifies 10x
High (power) dry objective - Correct Answers -40x
oil immersion objective - Correct Answers -100x magnification
What are the three distinct properties related to the functions of a microscope? - Correct
Answers -magnification
resolution
contrast
the number of times an image is enlarged - Correct Answers -magnification
striking difference between similar objects - Correct Answers -contrast
the ability of a lens system to show small, close together objects as being separate -
Correct Answers -resolution
What is some ways to increase contrast? - Correct Answers -staining
adjusting the light
what power is the oculars on a microscope? - Correct Answers -10x so for example if
you use the 4x magnification then you are seeing that image 40 times larger than
normal
the distance between the objective lens and the stage - Correct Answers -working
distance
The area visible through the microscope eyepiece - Correct Answers -field of view
the distance between the nearest and the furthest objects that give an image judged to
be in focus in a camera.
, the thickness of a plane of focus - Correct Answers -depth of field
maintain focus of a specimen as objective lenses are switched from one objective
power to another - Correct Answers -parfocal lens
What is used to minimize light scattering? - Correct Answers -oil immersion microscopy
or immersion oil
it has the same refractive index as glass. Light bends when it enters the glass and
remains of the same path as it passes through the immersion oil and enters the
objective
What objective is the only one that we can use oil? - Correct Answers -100x
the method of handling microbes and materials in a way that minimizes contamination -
Correct Answers -aseptic technique
the process of transferring a microbe from one medium to a next is called ------- and the
sample being transferred is called an ---- - Correct Answers -inoculation
inoculum
What two instruments are used to transfer an inoculum? - Correct Answers -inoculating
loop
inoculating needle
REREAD/LIST STEPS OF REMOVING INOCULUM FROM A LIQUID, SLANT, and
INOCULATING A BROTH AND PLATE - Correct Answers -Chapter 2
is a widely used method to separate individual bacteria from mixtures - Correct Answers
-isolation streak plate
What is the goal in an isolation streak? - Correct Answers -the goal of the streaking
pattern is to isolate individual bacteria from the mixture as a result of having fewer and
fewer bacteria as you continue around the plate
arises from a single bacterium that divides repeatedly over time to form a mass of a
millions of cells - Correct Answers -colony
in an isolation streak, small groups of cells come off the cell to form single ---- on the
surface of the plate - Correct Answers -isolated colonies
KNOW HOW TO DO LINES ON ISOLATION STREAK PLATE - Correct Answers -
chapter 3
KNOW/LABEL A MICROSCOPE - Correct Answers -chapter 1
Answers
father of hand hygiene - 1846 - Correct Answers -Ignaz Semmelweis
Ignaz Semmelweis, a Hungarian doctor working in Vienna General Hospital, is known
as the father of hand hygiene. In 1846, he noticed that the women giving birth in the
medical student/doctor-run maternity ward in his hospital were much more likely to
develop a fever and die compared to the women giving birth in the adjacent midwife-run
maternity ward. He decided to investigate, seeking differences between the two wards.
He noticed that doctors and medical students often visited the maternity ward directly
after performing an autopsy. Based on this observation, he developed a theory that
those performing autopsies got 'cadaverous particles' on their hands, which they then
carried from the autopsy room into the maternity ward. Midwives did not conduct
surgery or autopsies, so they were not exposed to these particles.
As a result, Semmelweis imposed a new rule mandating handwashing with chlorine for
doctors. The rates of death in his maternity ward fell dramatically. This was the first
proof that cleansing hands could prevent infection.
At a time when most people believed that infections were caused by foul odors called
miasmas, ----- implemented handwashing and other hygiene practices in the war
hospital in which she worked. While the target of these practices was to fight the
miasmas, her handwashing practices achieved a reduction in infections. - Correct
Answers -Florence Nightingale
Sadly, the hand hygiene practices promoted by Semmelweis and Nightingale were not
widely adopted. In general, handwashing promotion stood still for over a century. It was
not until the 1980s, when a string of foodborne outbreaks and healthcare-associated
infections led to public concern that the United States Centers for Disease Control and
Prevention identified hand hygiene as an important way to prevent the spread of
infection. In doing so, they heralded the first nationally endorsed hand hygiene
guidelines, and many more have followed.
How do you wash your hands? - Correct Answers -First wet hands and arms, apply
soap, turn off water and scrub for about 20 seconds (sing happy birthday twice) under
,nails and back of hands, use running water to rinse, and then dry either with paper towel
or air dry
What kind of microscope do we use in class? - Correct Answers -binocular compound
light microscope
What does binocular mean? - Correct Answers -it is used to describe microscopes with
two lenses
the compound microscope is designed for the examination of? - Correct Answers -
transparent specimens
scanning objective - Correct Answers -4x magnification
medium power objective - Correct Answers -magnifies 10x
High (power) dry objective - Correct Answers -40x
oil immersion objective - Correct Answers -100x magnification
What are the three distinct properties related to the functions of a microscope? - Correct
Answers -magnification
resolution
contrast
the number of times an image is enlarged - Correct Answers -magnification
striking difference between similar objects - Correct Answers -contrast
the ability of a lens system to show small, close together objects as being separate -
Correct Answers -resolution
What is some ways to increase contrast? - Correct Answers -staining
adjusting the light
what power is the oculars on a microscope? - Correct Answers -10x so for example if
you use the 4x magnification then you are seeing that image 40 times larger than
normal
the distance between the objective lens and the stage - Correct Answers -working
distance
The area visible through the microscope eyepiece - Correct Answers -field of view
the distance between the nearest and the furthest objects that give an image judged to
be in focus in a camera.
, the thickness of a plane of focus - Correct Answers -depth of field
maintain focus of a specimen as objective lenses are switched from one objective
power to another - Correct Answers -parfocal lens
What is used to minimize light scattering? - Correct Answers -oil immersion microscopy
or immersion oil
it has the same refractive index as glass. Light bends when it enters the glass and
remains of the same path as it passes through the immersion oil and enters the
objective
What objective is the only one that we can use oil? - Correct Answers -100x
the method of handling microbes and materials in a way that minimizes contamination -
Correct Answers -aseptic technique
the process of transferring a microbe from one medium to a next is called ------- and the
sample being transferred is called an ---- - Correct Answers -inoculation
inoculum
What two instruments are used to transfer an inoculum? - Correct Answers -inoculating
loop
inoculating needle
REREAD/LIST STEPS OF REMOVING INOCULUM FROM A LIQUID, SLANT, and
INOCULATING A BROTH AND PLATE - Correct Answers -Chapter 2
is a widely used method to separate individual bacteria from mixtures - Correct Answers
-isolation streak plate
What is the goal in an isolation streak? - Correct Answers -the goal of the streaking
pattern is to isolate individual bacteria from the mixture as a result of having fewer and
fewer bacteria as you continue around the plate
arises from a single bacterium that divides repeatedly over time to form a mass of a
millions of cells - Correct Answers -colony
in an isolation streak, small groups of cells come off the cell to form single ---- on the
surface of the plate - Correct Answers -isolated colonies
KNOW HOW TO DO LINES ON ISOLATION STREAK PLATE - Correct Answers -
chapter 3
KNOW/LABEL A MICROSCOPE - Correct Answers -chapter 1
