ASCP MLT CHEMISTRY EXAM LATEST ACTUAL EXAM
What is serum? - ANSWER: Serum is the fluid from blood without clotting factors
What is plasma? - ANSWER: Liquid portion of blood with clotting factors
Serum/Plasma which one is used for most chemistry tests - ANSWER: Serum is used
for most chemistry tests
_____ top tube is used for most chemistry tests - ANSWER: Red top tube is used for
most chemistry tests
Name four anticoagulants used in tubes for plasma - ANSWER: Oxalate, EDTA,
Citrate, and Heparin
What anticoagulant is the first choice for coagulation studies? - ANSWER: heparin is
first choice
Heparin on ice is used for what three tests? - ANSWER: Heparin (on ice): Ammonia,
Blood gasses, and lactic acid.
What preservative is used in a grey top tube? - ANSWER: Sodium fluoride is used as
a preservative in a grey top tube.
What does sodium fluoride do? - ANSWER: Sodium fluoride is used to preserve
glucose, aka slow glycolysis
T/F you can use a grey top for a BUN - ANSWER: FALSE the sodium fluoride in the
grey tube will inhibit urease
T/F you cant use a grey top for a BUN - ANSWER: TRUE the sodium fluoride would
inhibit urease
What anticoagulant contains Na+ and K+ - ANSWER: EDTA contains Na+ and K+
T/F you can test Na+ and K+ in a tube with EDTA - ANSWER: FALSE EDTA contains
Na+ and K+
T/F you can't test Na+ and K+ in a tube with EDTA - ANSWER: TRUE EDTA contains
Na+ and K+
T/F you cant test Ca++ in a tube with EDTA - ANSWER: TRUE because calcium is
chelated in EDTA (will cause a false lower result)
T/F you can test Ca++ in a tube with EDTA - ANSWER: FALSE because calcium is
chelated in EDTA (will cause a false lower result)
, Hemolysis: help KLAMP the leak means what? - ANSWER: When hemolysis occurs
the following lab results will elevate...
K- K+ (Potassium)
L- LD (Lactate Dehydrogenase)
A- ALD & ACP (Aldolase & Acid Phosphatase)
M- Mg++ (magnesium)
P- PO4 (Phosphate)
What happens to hemoglobin levels when a sample is hemolyzed? - ANSWER:
Hemoglobin levels will elevate during hemolysis
What is the end product of carbohydrates? - ANSWER: Glucose is the end product of
carbohydrates
What does insulin do to glucose levels? - ANSWER: Insulin decreases glucose levels
Glucose levels decrease approximately __ mg/dL per hour in whole blood (_%) -
ANSWER: Glucose levels decrease approximately 10 mg/dL per hour in whole
blood (7%)
Sodium fluoride ____ glycolysis - ANSWER: Sodium fluoride slows glycolysis
Fasting reference range for glucose in serum or plasma is ___-___mg/dL - ANSWER:
Fasting reference range for glucose in serum or plasma is 70-99mg/dL
"Normal" glucose CSF levels are approximately ___-____% of plasma glucose levels -
ANSWER: "Normal" glucose CSF levels are approximately 60-65% of plasma glucose
levels
What is the source of Insulin? - ANSWER: Insulin comes from the Beta cells of Islets
of Langerhens of pancreas
What is the source of Glucagon? - ANSWER: Glucagon comes from the alpha cells of
pancreas
What is the source of ACTH? - ANSWER: ACTH comes from the anterior pituitary
What is the source of growth hormone? - ANSWER: Growth hormone comes from
the anterior pituitary
What is the source of cortisol? - ANSWER: Cortisol comes from the adrenal cortex
What is the source of Human Placental Lactogen? - ANSWER: Human Placental
Lactogen comes from the Placenta
What is serum? - ANSWER: Serum is the fluid from blood without clotting factors
What is plasma? - ANSWER: Liquid portion of blood with clotting factors
Serum/Plasma which one is used for most chemistry tests - ANSWER: Serum is used
for most chemistry tests
_____ top tube is used for most chemistry tests - ANSWER: Red top tube is used for
most chemistry tests
Name four anticoagulants used in tubes for plasma - ANSWER: Oxalate, EDTA,
Citrate, and Heparin
What anticoagulant is the first choice for coagulation studies? - ANSWER: heparin is
first choice
Heparin on ice is used for what three tests? - ANSWER: Heparin (on ice): Ammonia,
Blood gasses, and lactic acid.
What preservative is used in a grey top tube? - ANSWER: Sodium fluoride is used as
a preservative in a grey top tube.
What does sodium fluoride do? - ANSWER: Sodium fluoride is used to preserve
glucose, aka slow glycolysis
T/F you can use a grey top for a BUN - ANSWER: FALSE the sodium fluoride in the
grey tube will inhibit urease
T/F you cant use a grey top for a BUN - ANSWER: TRUE the sodium fluoride would
inhibit urease
What anticoagulant contains Na+ and K+ - ANSWER: EDTA contains Na+ and K+
T/F you can test Na+ and K+ in a tube with EDTA - ANSWER: FALSE EDTA contains
Na+ and K+
T/F you can't test Na+ and K+ in a tube with EDTA - ANSWER: TRUE EDTA contains
Na+ and K+
T/F you cant test Ca++ in a tube with EDTA - ANSWER: TRUE because calcium is
chelated in EDTA (will cause a false lower result)
T/F you can test Ca++ in a tube with EDTA - ANSWER: FALSE because calcium is
chelated in EDTA (will cause a false lower result)
, Hemolysis: help KLAMP the leak means what? - ANSWER: When hemolysis occurs
the following lab results will elevate...
K- K+ (Potassium)
L- LD (Lactate Dehydrogenase)
A- ALD & ACP (Aldolase & Acid Phosphatase)
M- Mg++ (magnesium)
P- PO4 (Phosphate)
What happens to hemoglobin levels when a sample is hemolyzed? - ANSWER:
Hemoglobin levels will elevate during hemolysis
What is the end product of carbohydrates? - ANSWER: Glucose is the end product of
carbohydrates
What does insulin do to glucose levels? - ANSWER: Insulin decreases glucose levels
Glucose levels decrease approximately __ mg/dL per hour in whole blood (_%) -
ANSWER: Glucose levels decrease approximately 10 mg/dL per hour in whole
blood (7%)
Sodium fluoride ____ glycolysis - ANSWER: Sodium fluoride slows glycolysis
Fasting reference range for glucose in serum or plasma is ___-___mg/dL - ANSWER:
Fasting reference range for glucose in serum or plasma is 70-99mg/dL
"Normal" glucose CSF levels are approximately ___-____% of plasma glucose levels -
ANSWER: "Normal" glucose CSF levels are approximately 60-65% of plasma glucose
levels
What is the source of Insulin? - ANSWER: Insulin comes from the Beta cells of Islets
of Langerhens of pancreas
What is the source of Glucagon? - ANSWER: Glucagon comes from the alpha cells of
pancreas
What is the source of ACTH? - ANSWER: ACTH comes from the anterior pituitary
What is the source of growth hormone? - ANSWER: Growth hormone comes from
the anterior pituitary
What is the source of cortisol? - ANSWER: Cortisol comes from the adrenal cortex
What is the source of Human Placental Lactogen? - ANSWER: Human Placental
Lactogen comes from the Placenta
