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MB ASCP CONNECT EXAM ACTUAL EXAM WITH COMPLETE QUESTIONS AND ACCURATE ANSWERS RATED A+
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MB ASCP CONNECT EXAM ACTUAL EXAM WITH COMPLETE QUESTIONS AND ACCURATE ANSWERS RATED A+
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MB ASCP CONNECT 2024- 2025 EXAMACTUAL EXAM WITH COMPLETE
QUESTIONS AND ACCURATE ANSWERS
RATED A+
Which of the following is not involved in the splicing reaction?
a. 5' splice site
b. Hairpin loops
c. Branch A point
d. 3' splice site Correct Answer Hairpin loops
Which of the following statements are characteristics of the melt
curve analysis? (hint: more than one answer)
a. At the melting point, the probe separates from the target strand
and fluorescence rapidly decreases.
b. The melting temperature of double stranded DNA depends on
its base composition and length.
c. All PCR products for a specific primer pair should have the
same melting temperature.
d. When hybridization probes are utilized, the temperature is
incrementally decreased while fluorescence is monitored. Correct
Answer At the melting point, the probe separates from the target
strand and fluorescence rapidly decreases.
The melting temperature of double stranded DNA depends on its
base composition and length.
All PCR products for a specific primer pair should have the same
melting temperature.
,In the field of molecular diagnostics, which one of the following
genes is responsible for the synthesis of DNA, promote cell
division, and inhibit cell death?
a. Proto-oncogenes
b. Tumor suppressor genes
c. Oncogenes
d. Mitochondrial genes Correct Answer Proto-oncogenes
Microsatellite instability is best described as:
a. The ability of a gene with repeating elements of 8-10 base pairs
to randomly mutate
b. Contraction or expansion of the genome caused by frameshift
mutations (deletions or insertions) in elements of the genome
consisting of a repeating sequence of 1-3 base pairs
c. Repetitive elements in the genome with the ability to self-prime,
thus creating additional copies of genetic material at the allele loci
d. Random gene rearrangement between repeating elements of
the same size on different chromosomes resulting in different size
gene products Correct Answer Contraction or expansion of the
genome caused by frameshift mutations (deletions or insertions)
in elements of the genome consisting of a repeating sequence of
1-3 base pairs
Purines and pyrimidines differ from each other in that:
a. Purines are found RNA; pyrimidines are found in DNA
b. There's no difference between purines and pyrimidines
c. Pyrimidines have two rings; purines have one ring
d. Purines have two rings; pyrimidines have one ring Correct
Answer Purines have two rings; pyrimidines have one ring
TIP: Reciprocals of each other.
Purine, shorter word, longer rings.
,Pyrimidine, longer word, shorter ring.
Which of the following molecular methodologies would be the best
for detecting a trinucleotide repeat disorder such as Huntington's
disease?
a. Heteroduplex analysis
b. Variable number tandem repeat analysis
c. Single strand conformation polymorphism analysis
d. Reverse-Transcriptase PCR Correct Answer Variable number
tandem repeat analysis
Locus Test C M PF1 PF2
A1 12/15 12 15/17 12/15
B2 13 13/13 13/15 14/15
C3 7/8 8 7 7/7
D4 9/11 9/9 10/11 11
E5 6 6/6 5/7 6
Based on the results above, which one of the two possible fathers
is the two-month old's biological father?
a. Possible Father 1
b. Possible Father 2
c. Neither of the Possible Fathers Correct Answer Neither
Sickle cell disease is an autosomal genetic disease due to a point
mutation in the beta-globin gene, where glutamic acid is
substituted for valine at the sixth codon of the gene, resulting in a
faulty hemoglobin S (Hb S). Sickle cell disease is one of many
genetic diseases where a single gene controls the expression of
many phenotypic traits. The phenomenon where a single gene
controls the expression of many phenotypic traits is best referred
to as:
, a. Pleiotrophy
b. Polygenic inheritance
c. Epistasis
d. Epigenetics Correct Answer Pleiotrophy
A technologist uses the spectrophotometer to quantify the amount
of DNA extracted from a blood specimen diluted 1:30. The
absorbance reading at 260 nm was found to be 2.545. If the
absorbance at 280 nm gave a reading of 1.406, and if the the
DNA extract was re-suspended in 0.800 mL of EDTA solution, the
DNA yield is:
a. 3540 micrograms
b. 3450 micrograms
c. 3504 micrograms
d. 3054 micrograms Correct Answer 3054 ug
260 reading x 50 ug/ml (DNA) x dilution factor x resupsension =
DNA Yield
260 x 50 x 30 x 0.8 = 3054
When sequencing HLA-DR what is targeted?
a. Exon 1 of the α subunit
b. Exon 2 of the α subunit
c. Exon 1 of the β subunit
d. Exon 2 of the β subunit Correct Answer Exon 2 of the β
subunit
All of the following are components of nucleic acids, EXCEPT:
a. Phosphate group
b. Sugar (ribose or deoxyribose)
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