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MGG Exam 4/84 Questions and Answers 100% Scores
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MGG Exam 4/84 Questions andAnswers 100% Scores
A FASTA file always begins with a:
a. <
b. @
c. /
d. > - - D
- Besides the > sign, the first line of a FASTA file includes:
a. the read quality data
b. the sequence
c. the accession number for the sequence
d. the type of sequencing equipment used in obtaining the sequence - - C
- The second line of a FASTA file comprises:
a. the sequence
b. the read quality data
c. the accession number for the sequence
d. the type of sequencing equipment used in obtaining the sequence - - A
- A FASTQ file always begins with a:
a. <
b. >
c. @
d. / - - C
- The second line of a FASTQ file comprises:
a.the read quality data
b. the accession number for the sequence
c. the sequence
d. the type of sequencing equipment used in obtaining the sequence - - C
- The fourth line of FASTQ line comprises:
a. the accession number for the sequence
b. the type of sequencing equipment used in obtaining the sequence
c. the read quality data
d. the sequence - - C
- A PHRED score of 40 for a particular base call corresponds to the
probability of 1 base call error in how many base calls? [Note: this is a
Review Quiz question; you won't be asked to memorize the formula for the
exam]
a. 40
, b. 4,000
c. 10,000
d. 40,000 - - C
- What does a PHRED score of 30 or above indicate?
a. Low error rate for the entire read; acceptable quality
b. Low error rate for that base call; acceptable quality
c. High error rate for that base call; unacceptable quality
d. High error rate for the entire read; unacceptable quality - - B
- Which symbol represents a PHRED score of 36 in the Sanger FASTQ system
for using ASCII symbols to represent PHRED scores? [Note: this is a Review
Quiz question; you won't be asked to memorize the table in the exam]
a. $
b. E
c. @
d. m - - B
- Why are ASCII symbols used to represent PHRED scores in the Sanger
FASTQ system?
a. No answer text provided.
b. ASCII symbols have decimal places
c. ASCII symbols help with encryption of data
d. A two-character number can be represented by a one-character symbol - -
D
- What is crucial for assembling FASTQ files into longer stretches of genomic
sequence?
a. The FASTQ sequences must have overlap with neighboring sequences
b. The FASTQ sequences must be at least 10 kb long
c. No answer text provided.
d. The full genomic sequence must be already known - - A
- What is the purpose of sequencing just a single gene (16S rRNA) in DNA
extracted from a bacterial population in doing bacterial metagenomics?
a. To determine the metabolic potential of the population
b. To determine the abundance of each genus in the population
c. To determine the metabolic diversity of the population
d. To determine the size of the population - - B
- Why is the 16S rRNA sequence so commonly used for the metagenomics of
bacterial populations?
a. Only RNA sequence can be used for metagenomics
b. No answer text provided.
c. It is a common gene found in many species and has several highly
conserved sections
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