cbns 101 midterm 1 test questions & answers 2024/2025
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Course
Cbns 101
Institution
Cbns 101
cbns 101 midterm 1 test questions & answers 2024/2025
cell membrane permeability - ANSWERSsmall nonpolar molecules/ oxygen, small polar molecules/water, no ions, no large polar molecules
lipid bilayer properties - ANSWERScomposed of lipids and proteins. lipids are amphipathic making it ener...
cbns 101 midterm 1 test questions &
answers 2024/2025
cell membrane permeability - ANSWERSsmall nonpolar molecules/ oxygen, small polar molecules/water,
no ions, no large polar molecules
lipid bilayer properties - ANSWERScomposed of lipids and proteins. lipids are amphipathic making it
energetically favorable to spontaneously assemble into bilayers in aqueous solutions/hydrophobic
interior and hydrophilic heads interact with environment/leads to sealed rings of lipids
three types of lipids - ANSWERSphospholipid/fatty acids, cholesterol, glycolipid. each type is amphipathic
types of phospholipids - ANSWERShead group determines which. either derived from glycerol or
sphingosine/long tail very straight
membrane fluidity - ANSWERSextent to which lipid molecules can move in the plane of the membrane
cholesterol - ANSWERSthe shorter fatty acid tail intercalates between pls to prevent tight
binding/chaperone at lower temperatures in a confined space in the membrane
lipid rafts - ANSWERSthe accumulation of proteins in a given space in the membrane usually bound by
GPI anchor. sphingolipids and cholesterol involved. important for protein transport and cell signaling
plasma membrane as asymmetric structure - ANSWERSinner and outer leaflets have different lipid
concentrations, glycolipids are on external environment, cholesterol is distributed equally in both
leaflets, proteins distributed asymmetrically/fixed orientation
, lipid biosynthesis - ANSWERSoccurs only in the cytosolic leaflet of the ER. ER is the site of new
membranes being built, assembly site and transportation network, new membrane packed in vessicles
and gets shipped out into the cytosol
lipid synthesis - ANSWERSpl synthesis adds to cytosolic half of ER facing outside membrane, scramblase
enzyme flips pls so both halves of bilayer grow
to make lipids asymmetrical - ANSWERSflippase flips specific lipids to other leaflet, carbohydrates added
to to lipids and proteins only on the interior of ER
proteins arranged in membrane - ANSWERSthey are suspended directly in bilayer/float in sea of lipid.
they have distinct regions and can pass through the hydrophobic region of membrane
structure of membrane proteins - ANSWERSmembrane touching regions have hydrophobic side
chains/leucine/lysine so can interact/maximum hydrogen bonding of protein and lipid. peptide can loop
back and fourth through membrane making a defined orientation, alpha helix configuration. 3.6 AA per
turn of helix
GPI anchors - ANSWERSproteins covalently attaches to a glycolipid and anchor it in membrane. proteins
can only associate with external leaflet/outer surface of pm
FRAP - ANSWERSmonitors protein diffusion in membranes. fluorescent dye attached to lipids and
membrane proteins, bleach with laser/quenches excitation/big drop in intensity, recovery period, and
measure the amount of time it takes to recover fluorescence in the area
FRAP significance - ANSWERSdiffusion causes fluorescence to come back in which shows the fluidity of
the membrane
transport proteins - ANSWERSmove ions and small polar molecules across cell membranes through
aqueous channels/highly specific for which molecules they move
ion movement - ANSWERSdetermined by the concentration gradient on either side of the membrane
resulting in a membrane potential. movement of ions across membrane also influenced by electric
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