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CHM110H5 Chemical Principles 1 Pre-lab 1,2,3,5 QUIZ NEW UPDATE University of Toronto, Mississauga
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CHM110H5 Chemical Principles 1 Pre-lab 1,2,3,5 QUIZ NEW UPDATE University of Toronto, Mississauga
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CHM110H5 Chemical Principles 1 Pre-lab1,2,3,5 QUIZ NEW UPDATE University of
Toronto, Mississauga
, Experiment 1
Pre-lab Questions
1. Explain why it is important to wear gloves when handling the chromatography paper.
It is important to wear gloves when handling the chromatography paper because if not, the
paper can pick up substances that are on your fingertips, contaminating the paper and
altering the true results of the experiment.
2. What is the stationary phase, and what is the mobile phase in this experiment?
The stationary phase is the absorbent, absorbent paper, and the mobile phase is the
solvent that the paper is being placed into, 9ml of acetone and 1mL of 6M HCL .
3. What would happen in this experiment if the solvent level in the developing chamber
was more than 1.5cm high, i.e. if the sample spots were below the solvent level?
If the solvent level in the developing chamber was more than 1.5cm high and the sample
spots were below the solvent line, the sample spots would dissolve in the solvent. This
would prevent the spots from moving up, since there would not be any distinguished spots
for the compounds in the solutions to use to move up the paper using capillary action.
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