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Molecular - Chapter 4 - Nucleic Acid Extraction Questions and Answers
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Molecular - Chapter 4 - Nucleic Acid Extraction Questions and Answers Purpose of nucleic acid extraction to release nucleic acid (DNA or RNA) from the cell to use for subsequent procedures by lysing cell without damaging nucleic acid Target should be free from? contamination (protein, ...

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  • September 28, 2024
  • 9
  • 2024/2025
  • Exam (elaborations)
  • Questions & answers

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  • molecular chapter 4 nucleic acid extraction qu
  • purpose of nucleic acid extraction to release nucl
  • target should be free from contamination protein

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Molecular - Chapter 4 - Nucleic Acid
Extraction Questions and Answers

Purpose of nucleic acid extraction - answer to release nucleic acid (DNA or
RNA) from the cell to use for subsequent
procedures by lysing cell without
damaging nucleic acid

Target should be free from? - answer contamination (protein,
lipid, other nucleic acid)

Methods for DNA isolation - answer 1- organic,
2- inorganic, and
3- solid-phase

Methods for RNA isolation - answer 1-organic and
2-solid-phase

Steps of Nucleic Acid Extraction - answer 1-Lyse the cell to Release cellular
material;
2-Purify target nucleic acid;
3-Determine concentration and purity

why determining purity? - answer Quality control before performing the test.

Can be analyzed without
DNA amplification and why? - answer Blood
Buffy coat
Bone marrow
Solid tissue
Lavage fluids
Bacterial cultures
Fungi
Organelles, mitochondria.

Because quantity is sufficient.

What samples are adequate for amplification by PCR? - answer Dried blood;
Saliva;
Bone/teeth;
Amniotic fluid;
Hair follicles/hair shafts;

, Buccal cells;
Cerebrospinal fluids;
Fixed tissue;
Feces;
Soil.

Mitochondrial DNA is different from? - answer genomic DNA

Best fixed DNA specimens - answer Acetone;

10% buffered neutral formalin.

Not as good fixed DNA specimens - answer Zambonis, Clarkes
Paraformaldehyde
Formalin-alcohol-acetic acid
Metharcan

Less desirable fixed DNA specimens - answer Carnoys,

Zenkers,

Bouins,

B-5

Early DNA Extraction Methods - answer Density gradient centrifugation
Human DNA first isolated by Miescher in 1869

Organic dna extraction - answer uses organic chemicals, phenol, chloroform
(dissolve hydrophobic contaminants)

Organic chemicals for dna extraction - answer Phenol;

Chloroform.

Inorganic dna extraction - answer uses inorganic chemicals, detergents,
ethylenediamine tetraacetic acid (EDTA), acetic acid, salt (salting out, spooling)

Inorganic chemicals for dna extraction - answer detergents;

ethylenediamine tetraacetic acid (EDTA);

acetic acid;

salt (salting out, spooling)

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