Exam (elaborations)
Microbiology CSMLS Questions And Answers With Verified Solutions (GRADED A+)
- Course
- Institution
Microbiology CSMLS Questions And Answers With Verified Solutions (GRADED A+)
[Show more]
Content preview
Microbiology CSMLS Questions AndAnswers With Verified Solutions
(GRADED A+)
Microaerophiles - ANSWER✔✔ Prefer lower O2 than in air
Capnophiles - ANSWER✔✔ Prefer higher CO2 than in air
Agar - ANSWER✔✔ Polysaccharide derived from marine algae.
Melts @ 100c
Solidifies @ 45c
Anaerobic Media - ANSWER✔✔ Contains reducing agents that bind with
dissolved O2 (thioglycolate, cysteine)
Gas Pak - ANSWER✔✔ Contain Na2CO3 and Sodium borohydride
Add water - produces H2 and CO2
Palladium pellets catalyze the reaction
Typical Incubation - ANSWER✔✔ 5-10% CO2
35-37 c
50-70% humudity
,Autoclave - ANSWER✔✔ Moist - steam under pressure
15lbs pressure @ 121c for 15 minutes
QC organism - Bacillus stearothermophilus
Hot air oven - ANSWER✔✔ 170c for 2 hours
Pasteurization - ANSWER✔✔ 140c for 3 seconds.
NOT sterilization
Filtration - ANSWER✔✔ Pore size 0.22 u - 0.45 u
Used for sugar solutions, urea media, vaccines
Dessication - ANSWER✔✔ No multiplication, but organisms remain viable
(lyophilization)
Dry out bacteria
Phenol - ANSWER✔✔ Disinfection
Damages cytoplasmic membrane, denatures protein
,Halogens - ANSWER✔✔ Disinfection
Iodine and chlorine
Oxidizers
Alcohols - ANSWER✔✔ Disinfection
Denature protein and dissolve lipids
Sterilization - ANSWER✔✔ Kills all microorganisms (including spores and
viruses)
Eg.
- Autoclave (121c @ 15 psi for 15 min)
- Incineration
- Filtration (physically remove organisms)
Disinfection - ANSWER✔✔ Inactivation or inhibition (may not affect spores)
eg
- Bleach (1:10 hypochlorite)
B-lactams - ANSWER✔✔ Inhibits cell wall synthesis
, - Eg pencillins, cephalosporins, carbapenems, monobactams
Notes: Oxacillin, nafcillin, methicillin for pencillin resistant staph
Glycopeptides - ANSWER✔✔ Action: Inhibits cell wall synthesis
Examples: vancomycin
Notes: Drug of choice for clostridium difficile and MRSA
Aminoglycosides - ANSWER✔✔ Action: Inhibits protein synthesis
Examples: Gentamicin, tobramycin, amikacin
Notes: Acts on 30S subunit; not active against anerobes; used with a penicillin for
enterococcus
Tetracyclines - ANSWER✔✔ Action: Inhibits protein synthesis
Examples: tetracycline, doxycycline
Notes: Acts on 30S subunit; effects bone and teeth in children; may lead to
superinfection of yeast
Chloramphenicol - ANSWER✔✔ Action: Inhibits protein synthesis
The benefits of buying summaries with Stuvia:
Guaranteed quality through customer reviews
Stuvia customers have reviewed more than 700,000 summaries. This how you know that you are buying the best documents.
Quick and easy check-out
You can quickly pay through credit card or Stuvia-credit for the summaries. There is no membership needed.
Focus on what matters
Your fellow students write the study notes themselves, which is why the documents are always reliable and up-to-date. This ensures you quickly get to the core!
Frequently asked questions
What do I get when I buy this document?
You get a PDF, available immediately after your purchase. The purchased document is accessible anytime, anywhere and indefinitely through your profile.
Satisfaction guarantee: how does it work?
Our satisfaction guarantee ensures that you always find a study document that suits you well. You fill out a form, and our customer service team takes care of the rest.
Who am I buying these notes from?
Stuvia is a marketplace, so you are not buying this document from us, but from seller classhub. Stuvia facilitates payment to the seller.
Will I be stuck with a subscription?
No, you only buy these notes for $13.99. You're not tied to anything after your purchase.
Can Stuvia be trusted?
4.6 stars on Google & Trustpilot (+1000 reviews)
75860 documents were sold in the last 30 days
Founded in 2010, the go-to place to buy study notes for 14 years now