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Microbiology CSMLS Questions And Answers With Verified Solutions (GRADED A+)
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Microbiology CSMLS Questions And Answers With Verified Solutions (GRADED A+)
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Microbiology CSMLS Questions AndAnswers With Verified Solutions
(GRADED A+)
Microaerophiles - ANSWER✔✔ Prefer lower O2 than in air
Capnophiles - ANSWER✔✔ Prefer higher CO2 than in air
Agar - ANSWER✔✔ Polysaccharide derived from marine algae.
Melts @ 100c
Solidifies @ 45c
Anaerobic Media - ANSWER✔✔ Contains reducing agents that bind with
dissolved O2 (thioglycolate, cysteine)
Gas Pak - ANSWER✔✔ Contain Na2CO3 and Sodium borohydride
Add water - produces H2 and CO2
Palladium pellets catalyze the reaction
Typical Incubation - ANSWER✔✔ 5-10% CO2
35-37 c
50-70% humudity
,Autoclave - ANSWER✔✔ Moist - steam under pressure
15lbs pressure @ 121c for 15 minutes
QC organism - Bacillus stearothermophilus
Hot air oven - ANSWER✔✔ 170c for 2 hours
Pasteurization - ANSWER✔✔ 140c for 3 seconds.
NOT sterilization
Filtration - ANSWER✔✔ Pore size 0.22 u - 0.45 u
Used for sugar solutions, urea media, vaccines
Dessication - ANSWER✔✔ No multiplication, but organisms remain viable
(lyophilization)
Dry out bacteria
Phenol - ANSWER✔✔ Disinfection
Damages cytoplasmic membrane, denatures protein
,Halogens - ANSWER✔✔ Disinfection
Iodine and chlorine
Oxidizers
Alcohols - ANSWER✔✔ Disinfection
Denature protein and dissolve lipids
Sterilization - ANSWER✔✔ Kills all microorganisms (including spores and
viruses)
Eg.
- Autoclave (121c @ 15 psi for 15 min)
- Incineration
- Filtration (physically remove organisms)
Disinfection - ANSWER✔✔ Inactivation or inhibition (may not affect spores)
eg
- Bleach (1:10 hypochlorite)
B-lactams - ANSWER✔✔ Inhibits cell wall synthesis
, - Eg pencillins, cephalosporins, carbapenems, monobactams
Notes: Oxacillin, nafcillin, methicillin for pencillin resistant staph
Glycopeptides - ANSWER✔✔ Action: Inhibits cell wall synthesis
Examples: vancomycin
Notes: Drug of choice for clostridium difficile and MRSA
Aminoglycosides - ANSWER✔✔ Action: Inhibits protein synthesis
Examples: Gentamicin, tobramycin, amikacin
Notes: Acts on 30S subunit; not active against anerobes; used with a penicillin for
enterococcus
Tetracyclines - ANSWER✔✔ Action: Inhibits protein synthesis
Examples: tetracycline, doxycycline
Notes: Acts on 30S subunit; effects bone and teeth in children; may lead to
superinfection of yeast
Chloramphenicol - ANSWER✔✔ Action: Inhibits protein synthesis
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