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Transfusion CSMLS Questions And Answers With Verified Solutions Already Passed!!!
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Transfusion CSMLS Questions And Answers With Verified Solutions Already Passed!!!
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Transfusion CSMLS Questions AndAnswers With Verified Solutions Already
Passed!!!
Kleihauer Betke Test - ANSWER✔✔ If Rosette test is positive, quantifies FMH.
Acid elution principle, fetal hemoglobin (HgbF) is resistant to acid elution, adult
hemoglobin (HgbA) is not resistant.
-Quantitative test
1. Blood smear from postpartum maternal sample exposed to acid buffer.
2. HgB from adult red cells leaches into buffer and leaves only stroma, whereas the
fetal red cells retain their Hgb
3. Smears washed, stained and examined under oil immersion.
4. Adult red cells appear as "ghosts", fetal cells appear pink
Reported as percentage of fetal red cells ( # fetal red cells / total red cell count)
#fetal cells/1000 RBC
Rosette Test - ANSWER✔✔ Screen method , demonstrates presence of Rh
Positive Baby cells.
- Qualitative test
Suspension of maternal Red cells (contains D -negative maternal and small # of D-
positive fetal red cells) incubated with Anti-D. Anti-D binds to D-pos fetal cells.
,Suspension then washed and D positive indicator red cells added which bind to
anti-D and form rosette around D positive fetal red cells, which is then examined
microscopically.
< 1 rosette per 3 lpf: 1 dose of RhIG> 1 rosette per 3pg: quantitate bleed
ACD, CPD, CP2D - ANSWER✔✔ 21 days after used (Anticoagulants)
CPDA-1 - ANSWER✔✔ Anticoagulant
35 day expiration
Additive Solutions - ANSWER✔✔ Preserving solutions that are added to the
RBCs after removal of the plasma with or without platelets
42 days
Packed Red Blood Cells (PRBCs) - ANSWER✔✔ Red cells with plasma removed
To increase oxygen-carrying capacity in patients with anemia, in patients with
substantial hemoglobin deficits, and in patients who have lost up to 25% of their
total blood volume
Washed Red Cells - ANSWER✔✔ - Plasma removed by successive saline washes
(automated instruments)
- Prevents allergic response to plasma proteins and anaphylactic shock in IgA
deficient patients with anti-IgA (IgA is in normal plasma)
- Expires 24 hours after seal of original unit broken
, Leukocyte reduced RBCs - ANSWER✔✔ Removal of leukocytes (WBCs) from
the RBC component resulting in <5 x 10^6 WBCs in the entire unit.
85% of RBCs retained.
- For patients with repeated febrile nonhemolytic FNH reactions.
Deglycerolized RBCs - ANSWER✔✔ Cryoprotective agent (40% glycerol) protect
from ultra low temperatures.
- Thawed @ 37c and glycerol removed. 80% of RBCs must be recovered.
- Stored @ <-65c; 1-6c for 24hrs after deglycerolizing
- Used for storage of autologous units and rare units; expires in 10 years.
Fresh Frozen Plasma (FFP) - ANSWER✔✔ -Prepared by separating cells and
plasma by centrifugation and freezing plasma within 8 hours of collection
-Expires 1 year from date of collection when stored at ≤ 18°C or 7 years stored at ≤
-65°C
-Once thawed (between 30-37°C), expires in 24 hours, if stored at 1-6°C
- Used for multiple coagulation deficiencies, factor XI deficiency and other
congenital deficiencies for which no concentrate is available
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