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CSMLS - Hematology Lab Manual Questions And Answers With Verified Solutions 100% Correct!!!
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CSMLS - Hematology Lab Manual Questions And Answers With Verified Solutions 100% Correct!!!
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CSMLS - Hematology Lab ManualQuestions And Answers With Verified
Solutions 100% Correct!!!
Cytospin used for fluids with _ number of cells (4) - ANSWER✔✔ low
synovial, pleural, pericardial, CSF
Angle for PBS smear - ANSWER✔✔ 30
A film can cause _ in smear
Clean with _ wash, dH2O, _ - ANSWER✔✔ holes
soapy/methanol
For cell morphology maintenance make smear within _ hours of collection
Abnormal cell distribution caused by _ of drop before smearing - ANSWER✔✔ 3
drying
Uneven motion causes _
Lifting spreader causes _
Uneven pressure causes _ - ANSWER✔✔ waves
tails
chatters/flat sides
<30 angle smear
>30 angle smear - ANSWER✔✔ long/thin (large monolayer)
, short/thick (small monolayer)
Cause of false increase in lymphocyte count - ANSWER✔✔ long/thin smear push
mono/neuts to feathered edge
Hepatitis & HIV are _ _ by methanol fixation - ANSWER✔✔ not inactivated
Romanowsky stain contains _ _ (or its products of _) & a _ _ dye (2)
3 examples called _ stains - ANSWER✔✔ methylene blue/oxidation/halogenated
fluorescent (Eosin B or Y)
Wrights, Giemsa, Leishmans
polychrome
Eosin is _ and stains _ components a _ colour
Methylene blue/azures is _ and stains _ components a _ colour
Neutral cell components are stained by _ and have a _ colour - ANSWER✔✔
acidic/basic (Hgb/eosin granules)/pink
basic/acidic (DNA/granules)/blue
basic & acidic/pink-lilac
Methanol fixation purpose (5) - ANSWER✔✔ kill RBC
change refractive index of cell
renders more cell more permeable
cause cell to adhere to glass slide
dehydrates cell
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