BIOL 2460 LAB PRACTICAL 2 QUESTIONS AND CORRECT ANSWERS
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Course
BIO 2460
Institution
BIO 2460
A bacterial species that cannot tolerate the presence of oxygen and will only grow in the absence of oxygen is classified as
a. obligate aerobe b. obligate anaerobe c. facultative aerobe d. mcroaerophile e. aerotolerant anaerobe b
A bacterial isolate is subcultured to two blood agar plates, one o...
BIOL 2460 LAB PRACTICAL 2
QUESTIONS AND CORRECT ANSWERS
A bacterial species that cannot tolerate the presence of oxygen and will only grow in the
absence of oxygen is classified as
a. obligate aerobe
b. obligate anaerobe
c. facultative aerobe
d. mcroaerophile
e. aerotolerant anaerobe ✅b
A bacterial isolate is subcultured to two blood agar plates, one of which is incubated in
an anaerobe jar, and the other is incubated on a shelf in the incubator. After incubation,
growth is observed on the plate from the anaerobe jar, but there is no growth on the
plate incubated on the incubator shelf. Based on these results, how would this organism
be categorized?
a. obligate aerobe
b. obligate anaerobe
c. aerotolerant anaerobe
d. facultative anaerobe ✅b
Where would you predict an obligate anaerobe would grow in a tube of fluid
thioglycollate medium?
a. at or very near the surface of the broth
b. a little below the surface of the broth
c. throughout the entire tube
d. at the bottom of the tube ✅d
The pink color in the top of the broth in an FTM tube indicates
a. the absence of oxygen
b. the presence of carbon dioxide
c. the presence of oxygen
d. the presence of thioglycollate ✅c
Taq polymerase is a DNA polymerase enzyme that demonstrates maximal activity at 72
C. The organism from which this enzyme was isolated would most likely be classified as
a:
a. psychrotroph
b. psychrophile
, c. mesophile
d. thermophile
e. hyperthermophile ✅d
Both human pathogens and normal microbiota are typically classified as
a. psycrophiles
b. thermophiles
c. mesophiles
d. hyperthermophiles ✅c
The higher the pH the more ___ the environment.
a. acidic
b. alkaline
c. neutral
d. hypotonic ✅b
Acetobacter aceti is a bacterial species involved in the production of acetic acid during
vinegar production. Which category would this
a. neutrophile
b. acidophile
c. akalinophile ✅a
Microorganisms that can grow at salt concentrations as high as 15-30% are known as
a. halotlerant
b. halophiles
c. hypertoniphiles
d. osmophlles ✅b
An experiment involves the inoculation bacteria into a variety of media with NaCl
concentrations ranging from 1% to 25%
a. 1% NaCl
b. 5% NaCl
c. 10% NaCl
d. 25% NaCl ✅a
Plasmolysis is the ___ of a bacterial cell in a ___ solution.
a. swelling...hypertonic
b. swelling...hypotonic
c. shrinking...hypertonic
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