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CGMBS EXAM 5 (2024) QUESTIONS WITH PASSED SOLUTIONS!!
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CGMBS EXAM 5 (2024) QUESTIONS WITH PASSED SOLUTIONS!!
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CGMBS EXAM 5(2024)
QUESTIONS
WITH PASSED
SOLUTIONS!!
Evatee 10/2/24 CGMBS
, CGMBS EXAM 5 (2024) QUESTIONS WITH
PASSED SOLUTIONS!!
What does SPRI stand for? Answer - Solid Phase Reversible Immobilization
How does the ratio of SPRI to product select for a particular fragment size?
Answer - Increasing the ratio of SPRI to DNA will increase the recovery of small
DNA fragments
Decreasing the ratio of SPRI to DNA will result in less recovery of small
fragments
What are sequence adapters? Answer - Sequence adapters are unique sample
barcodes (i5 and i7)
What do sequence adapters bind to? Answer - They anneal to the A-based end
and ligase enzyme repairs the nick in the phosphate backbone of the DNA
molecule
The A overhang allows adapters containing a single thymine overhanging base
to pair with the DNA fragment
What are the steps in the watchmaker workflow? Answer - Watchmaker
consists of:
1. Frag A-tailing (Frag enz and Frag buff)
2. Ligation of adapters
3. SPRI clean up
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